Basic Informations
C.V
Samar sayed ewies
Email address: samarewies@yahoo.co.uk
Mobile no.: +201095327112
Work phone: +2 082 2322066
Fax no. : +2 082 2327982
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Personal Profile
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Date of Birth: 3/10/1990
Gender: Female
Nationality: Egyptian
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Field of specialization
Affiliation
Current Position
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Virology
Virology department, Veterinary Medicine college, Beni-Suef University, Beni-Suef 62511, EGYPT.
Demonstrator of virology
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Education
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2017 M.Sc Beni-Suef University, Egypt.
2012 B. Sc Beni-Suef University, Egypt.
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Work Experience
Teaching activities
Training programs
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2013-2017 Demonstrator of virology
At Veterinary Medicine college, Beni-Suef University, EGYPT.
? From 2013 – 2017 teaching the practical course of virology for the under-graduate (third year) and post-graduate students in Faculty of Veterinary Medicine Beni-Suef University, Egypt
? Training cycle in University Management from Faculty and Leadership Development Project (FLDP).
? Training cycle in university legal and financial aspects from Faculty and Leadership Development Project (FLDP).
? Training cycle in competitive research projects from Faculty and Leadership Development Project (FLDP).
? Training cycle in international publishing of research from Faculty and Leadership Development Project (FLDP).
? Training cycle in the Quality Standards in Teaching from Faculty and Leadership
Development Project (FLDP).
? Training cycle in organizing scientific conferences from Faculty and Leadership Development Project (FLDP).
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Research: Molecular characterization of Newcastle disease virus (genotype VII) from broiler chickens in Egypt
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Master Title
Molecular characterization of recent isolates of Newcastle disease virus in Egypt"
Master Abstract
Summary
Newcastle disease (ND) is one of the most devastating diseases affecting poultry especially in large scale production causing severe economic losses due to high mortality which may reach 100% in very virulent strains of NDV.
NDV is (A) listed disease in the OIE Terrestrial Animal Health Code. However, not all NDV strains are virulent, thus this classification based on the virus characteristics.
In the current study, characterization of some Newcastle disease virus (NDV) isolates from different localities and different years in Egypt carried out.
Virus isolation was attempted through inoculation in allantoic sac of 10-day-old SPF-ECE using tissue samples collected from 68 farms.
All allantoic fluids harvested from the inoculated eggs were subjected to plate haemagglutination test (HA) out of 68 samples 31 giving positive results .The isolated viruses were identified by HI test using NDV-specific antisera proving the success of isolation of NDV.The HI results showed 31positive samples out of 68 tested samples .
RT-PCR was used for the detection of partial F-gene of vNDV. Partial fusion gene sequence of 4 isolates which were of velogenic type revealed that (112RRQKRF117) dibasic cleavage site sequence and Phylogenetic analysis of the sequenced part of fusion protein and comparison with other reference strains from Gene Bank revealed that isolates are close to genotype VII of Chinese origin.
The recent vNDV isolates in the study (gene bank acc.no. KY693681-KY693684) showed complete identity to each other with 100% identity and with other strains isolated during 2011-2014 the identity ranged between 94.1-100% that denotes to minor genetic variation.
In conclusion, the study presents the molecular characterization of 4 NDV isolates in Egypt. Data obtained from the study confirm the Partial F gene sequence analysis showed that the isolated NDV strains belong to genotype VII with the characteristic amino acid sequences of the F0 protein proteolytic cleavage site motifs (112RRQKRF117) for the velogenic NDV strains.
PHD Title
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PHD Abstract
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