Basic Informations
C.V
Beni-Sueif University
Faculty of Veterinary Medicine
Department of bacteriology, mycology and immunology
Curriculum vitae
PERSONAL DETAILS:
Name: Amr Shehata Mohammed Ramadan
Date of birth: 4/9/1991
Place of birth: Alzawya Alkhadraa - Sinnuris - El-Fayoum
Country of birth: Egyp?Nationality: Egyptian
Correspondence address Faculty of vet. Med. Beni-Suef University.
Social status Single
Tel. No. 01028980874
Fax No. 020822327982
E-mail vet.amr.shehata.2013@Gmail.com
amrshehata_91@vet.bsu.edu.eg
ACDADEMIC DETAILS
HIGHER EDUCATION1. Bachelor in Veterinary Medicine (B. V. Sc.) Beni-Suef Univ., 2013 with a degree of very good with honor.
2. Master of Microbiology and Immunology, College of Veterinary Medicine, Beni-Suef University 2017.
Position:
1. Demonstrator in Microbiology Dept. in 5/3/2015
Responsibility:
1. Teaching practical of Bacteriology, Mycology and Immunology to 3rd year undergraduates of the faculty.
Master Title
Phenotypic and genotypic characterization of coagulase negative Staphylococcus species isolated from mastitic cattle
Master Abstract
Bacteriological examination of 364 quarter milk samples from a total of 253 cows reacted positive with California mastitis test was done for isolation and identification of CNS causing bovine subclinical mastitis cases. A total of 213 staphylococcus isolates were recovered then by using coagulase test it's found that 95 isolates were coagulase negative in a prevalence of 26.09%. Using api® staph kits to further identify the recovered CNS isolates revealed that S. xylosus (36.48%), S. chromogenes (12.63%), S. epidermidis (10.53%), S. saprophyticus (8.42%) and then S. haemolyticus (7.38%) were the most predominant among a total of 13 Staphylococcus species were identified. The in vitro susceptibility test using disk diffusion method against 14 antimicrobial agents revealed that 100% of the CNS were sensitive to Imipenem, while the majority of isolates were sensitive to Ciprofloxacin (96.84%), Oflxacin (92.63%), Vancomycin (85.26%), Chloramphenicol (85.26%), amoxycillin-clavulanic acid (83.16%) and sulfamethoxazole-trimethoprim (80%). While, 95.79% were resistant to ampicillin, 77.9% to cefoxitin, 35.79% to cefuroxime, 31.58% to amoxycillin and 18.95% were resistant to clindamycin. Multidrug resistance (MDR) was detected in 60% (n=57) of isolates, methicillin resistance (MR) was identified in 77.9% (n=74) and vancomycin resistance was detected in 9.47% (n=9) of isolates. Using PCR for detection of 5 resistance genes among selected MDR isolates revealed existence of kan gene in 30%, blaZ in 50% and in 70% in case of mecA, ermC and tetK genes. Antimicrobial effect of 2 essential oils; cinnamon and carvacrol was studied on MDR isolates and it's was found that cinnamon oil (0.1% concentration) inhibited 62.5% of tested isolates, whereas carvacrol (0.1% concentration) was totally inhibitory to all tested isolates, while 0.01% and 0.001% concentrations of the 2 oils were of no inhibitory effect at all.
Biofilm (Slime) formation capacities, a very important virulence marker in Staphylococcus species, was assessed phenotypically on Congo red agar plates in recovered CNS and it was detected in 47.4%. Using PCR to assess 2 biofilm genes; icaD and bap in 10 random MDR showed a prevalence of 10% for each gene.
PHD Title
PHD Abstract