Basic Informations

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Mohamed Abdelgied
Date of Birth:	October 10, 1987
Nationality :	Egyptian
Gender :	Male
Affiliations :	-PhD student (Expected graduation September, 2019), Department of Experimental Pathology and Tumor Biology, Graduate school of Medical sciences, Nagoya city university, Japan. -Nano material toxicology laboratory, Nagoya city university, Japan. - Toxicology department, School of veterinary medicine, Beni-suef university, Egypt.
Mailing address :	Nagoya City University Graduate School of Pharmaceutical Sciences 3-1 Tanabedohri, Mizuho-ku, Nagoya 467-8603, Japan. Tel: +81 52 836 3496 Tel & Fax: +81 52 836 3497 E-mail: c151902@ed.nagoya-cu.ac.jp m.abdelgaid@vet.bsu.edu.eg abdelgiedm@yahoo.com
Education:
2009, Bc.V.Sc.	Beni-suef university, school of veterinary medicine, Egypt.
2010-2012, Ms.V.Sc.	Toxicology and Forensic medicine department, School of veterinary medicine, Beni-suef university, Egypt.
2015- New	PhD student, Department of Experimental Pathology and Tumor Biology, Graduate school of Medical sciences, Nagoya city university, Japan.
Positions Held
2010-2012	Demonstrator, Toxicology and Forensic medicine department, School of veterinary medicine, Beni-suef university, Egypt.
2013-2015	Assistant lecturer, Toxicology and Forensic medicine department, School of veterinary medicine, Beni-suef university, Egypt.
Teaching activities, 2010-2015
- Teaching practical courses of Veterinary Forensic medicine and Toxicology for fourth year students in the Faculty of Veterinary Medicine Beni-Suef University. - Participation in summer training courses for fourth year students. - Participation in analysis of samples in the atomic absorption spectrophotometry unit of the Faculty of Veterinary Medicine, Beni-Suef University. - Participating in the organization of workshops held in the department. - Participating in the practical part of theses registered in the department.
Membership of Scientific Organizations:
2017- Now	Japanese Society of Toxicological Sciences
2017- Now	Japanese Society of Toxicologic Pathology
2010- Now	Egyptian Society of Environmental toxicology
Recent Main Research Interests:
Nanomaterial and ultrafine material toxicology, mainly engineered titanium dioxide and carbon nanomaterials.
Publications: - Mohamed Abdelgied, Ahmed M. El-Gazzar, William T.Alexander, Takamasa Numano , Masaaki Iigou , Aya Naiki-Ito , Hiroshi Takase , Akihiko Hirose , Yuhji Taquahashi , Jun Kanno , Mona Abdelhamid, Khaled Abbas Abdou , Satoru Takahashi , David B. Alexander , and Hiroyuki Tsuda: Carcinogenic effect of potassium octatitnate (POT) fibers in the lung and pleura of male Fischer 344 rats after intrapulmonary administration. Submitted to Particle and fiber toxicology. - Takamasa Numano, Hitomi Higuchi, David Alexander, William Alexander, Mohamed Abdelgied, Ahmed ELGazzar, Dina Saleh, Hirotsugu Takase, Akihiko Hirose, Aya Naiki-Ito, Shugo Suzuki, Satoru Takahashi, Hiroyuki Tsuda: MWCNT-7 administered to the lung by intratracheal instillation induces development of pleural mesothelioma in F344 rats. Cancer Sci. 2019 Jul 2. doi: 10.1111/cas.14121. [Epub ahead of print]. - Mohamed Abdelgied, Ahmed M. El-Gazzar, David B. Alexander , William T. Alexander, Takamasa Numano, Masaaki Iigo, Aya Naiki, Hiroshi Takase, Akihiko Hirose, Jun Kannno, Mona Abdelhamid, Hiroyuki Tsuda, Satoru Takahashi: Pulmonary and pleural toxicity of potassium octatitanate fibers, rutile titanium dioxide nanoparticles, and MWCNT-7 in male Fischer 344 rats. Archives of Toxicology, doi.org/10.1007/s00204-019-02410-z, February 2019. - Ahmed M. El-Gazzar, Mohamed Abdelgied, David B. Alexander , William T. Alexander, Takamasa Numano, Masaaki Iigo, Aya Naiki, Satoru Takahashi, Hiroshi Takase, Akihiko Hirose, Jun Kannno, Osama Saeid Elokle, Ashraf Mohamed Nazem, Hiroyuki Tsuda: Comparative pulmonary toxicity of a DWCNT and MWCNT-7 in rats. Archives of Toxicology doi.org/ 10.1007/s00204-018-2336-3, October 2018.
- Dongping Liao, Qiqi Wang, Jiali He, David Alexander, Mohamed Abdelgied, Ahmed El-Gazzar, Mitsuru Futakuchi, Masumi Suzui, Jun Kanno, Akihiko Hirose, Jiegou Xu, Hiroyuki Tsuda: Persistent pleural lesions and inflammation by pulmonary exposure of multi-walled carbon nanotubes. Chem. Res. in Toxicol., 31(10), 1025-1031, October 2018. - Mohamed Abdelgied, Ahmed M. El-Gazzar, David B. Alexander, William T. Alexander, Takamasa Numano, Masaaki Iigo, Aya Naiki, Hirotsugu Takase, Akihiko Hirose, Yuhji Taquahashi, Jun Kanno, Hiroyuki Tsuda and Satoru Takahashi: Potassium octatitanate fibers induce persistent lung and pleural injury and are possibly carcinogenic in male Fisher 344 rats. Cancer Sci., doi:10.111/cas.13643, May 2018. - David B. Alexander, Masaaki Iigo, Mohamed Abdelgied, Keiji Ozeki, Satoshi Tanida, Takashi Joh, Satoru Takahashi, and Hiroyuki Tsuda: Bovine lactoferrin and Crohn's Disease: A case study. Biochemistry and Cell Biology. 95(1): 133-141; Feb 2017. - Masumi Suzui, Mitsuru Futakuchi, Katsumi Fukamachi, Takamasa Numano, Mohamed Abdelgied, Satoru Takahashi, Makoto Ohnishi, Toyonori Omori, Shuji Tsuruoka, Akihiko Hirose, Jun Kanno, Yoshimitsu Sakamoto, David B. Alexander, William T. Alexander, Xu Jiegou and Hiroyuki Tsuda: Multiwalled carbon nanotubes intratracheally instilled into the rat lung induce development of pleural malignant mesothelioma and lung tumors. Cancer Sci. 107(7): 924-35 doi: 10.1111/cas.12954, April 2016. - H. Tsuda, D.B. Alexander, W.T. Alexander, M. Abd Elgied, A. Elgazzar, J. Xu1, T. Numano, M. Suzui, M. Futakuchi, K. Fukamachi, A. Hirose, J. Kanno (2016) Development of a mechanism based short-term assay protocol to test carcinogenicity of multiple wall carbon nanotubes (MWNCTs) in the rat. Toxicology letters. Master thesis: Ameliorative role of green tea extract and Alpha lipoic acid against sub chronic copper nanoparticles induced toxicity in rats.
International conferences and meetings - Hiroyuki Tsuda, William. Alexander, Takamasa Numano, Mohamed Ahmed Mahmoud Abdelgied, Ahmed Maher Mahmoud Ibrahim Elgazzar, Hiroshi Takase, Jiegou Xu, Aya Naiki, Satoru Takahashi, Akihiko Hirose, Makoto Ohnishi, Jun Kanno, and David B Alexander: Demonstration of the carcinogenicity of a thin multi-walled carbon nanotube in the rat lung. Poster presentation. American Association for Cancer research meeting (Environmental Carcinogenesis: Potential Pathway to Cancer Prevention). June 22-24, 2019; Charlotte, North Carolina, USA. - M.A.M. Abdelgied, A. El-Gazzar, D. Alexander, W. Alexander, T. Numano, M. Iigo, A. Naiki, M. Abdelhamid, H. Takase, A. Hirose, Y. Taquahashi, J. Kanno, S. Takahashi, H. Tsuda: Potassium octatitanate fibers are possibly carcinogenic in male Fischer 344 rats. Toxicology Letters 295S (2018). 54^th Congress of the European Societies of Toxicology September 2–5, 2018, Brussels, Belgium.
- Mohamed Abdelgied, Ahmed M. El-Gazzar, David B. Alexander, William T. Alexander, Takamasa Numano¹, Masaaki Iigo, Aya Naiki, Hirotsugu Takase, Khaled Abbas Abdou, Akihiko Hirose, Yuhji Taquahashi, Jun Kannno, Satoru Takahashiand Hiroyuki Tsuda: Potassium octatitanate (K₂O.8TiO₂) fiber is a potent inducer of lung and pleural injury in male Fischer 344 rats: A comparative study of titanium dioxide nano particles. Poster presentation, The 34^th Annual Meeting of the Japanese Society of Toxicologic Pathology; Janurary 25-26, 2018; Okinawa, Japan. - Ahmed M. El-Gazzar, Mohamed Abdelgied, David B. Alexander, William T. Alexander, Takamasa Numano1, Masaaki Iigo, Aya Naiki, Hirotsugu Takase, Akihiko Hirose, Yuhji Taquahashi, Jun Kannno, Satoru Takahashi, Ashraf Mohamed Nazem, Osama Saeid Elokle, Hiroyuki Tsuda: Comparative Pulmonary toxicity of DWCNT and MWCNT-7 in Rats. Poster presentation. The 34^th Annual Meeting of the Japanese Society of Toxicologic Pathology; Janurary 25-26, 2018; Okinawa, Japan. -Mohamed Abdelgied, Ahmed M. El-Gazzar, David B. Alexander, William T. Alexander, Takamasa Numano, Masaaki Iigo, Aya Naiki, Hirotsugu Takase, Khaled Abbas Abdou, Akihiko Hirose, Yuhji Taquahashi, Jun Kannno, Satoru Takahashiand Hiroyuki Tsuda: Potassium Octa,tanate Fiber (K₂O.8TiO₂) is a Potent Inducer of Lung and Pleural Injury - A Compara,ve Study to Titanium Dioxide Nano Particles. Oral Presentation; 45^th Annual meeting of Japanese society of toxicology; Jul 10-12, 2017; Yokahama, Japan. - Hiroyuki TSUDA, Jiegou XU, William T ALEXANDER, David B ALEXANDER, Mohamed ABDELGIED, Ahmed ELGAZZAR, Takamasa NUMANO, Akihiko HIROSE, Jun KANNO: Trans-tracheal Intra-pulmonary Spraying Method (TIPS) for the Evaluation of Respiratory Neoplasia Development by Carbon Nanotubes. 45 Annual meeting of Japanese society of toxicology; Jul 10-12, 2017; Yokahama, Japan. - David B. Alexander, Masaaki Iigo, Mohamed Abdelgied, Keiji Ozeki, Satoshi Tanida, Takashi Joh, Satoru Takahashi, and Hiroyuki Tsuda: Bovine lactoferrin and Crohn's Disease: A case study; XII ^th lactoferrin conference, November, 2015, Nagoya, Japan. - Heavy metals risk assessment workshop, Oct 2013,Beni-suef, Egypt. - Organizer: 5 ^th International Conference of The Egyptian Society of Environmental Toxicology "Towards Safe Food in Egypt", March 2013; Beni Suef, Egypt. - Workshop entitled “Risk assessment as a critical tool for everyday challenges” that was held in cooperation with Environmental Protection Agency, United States of America (EPA-USA), 17-19 May, 2012; Cairo, Egypt.
Research Fellow:
- Fully funded PhD studying program from Egyptian Ministry of higher education.
Current work - Currently I’m investigating the long-term consequences in rats of intrapulmonary administration of (1) different doses (0.125 mg, 0.25 mg, 0.50 mg) of one type of DWCNT; (2) different lengths (1 µm, 7 µm, 15 µm) of another type of DWCNT; (3) potassium octatitanate and MWCNT-7 fibers dispersed by two different methods.

C.V PDF

Master Title

Ameliorative role of some antioxidants against sub chronic copper toxicity in rats.

Master Abstract

The rapid growth of nanotechnology suggests that it will soon find wide application in daily consumer products and new pharmaceutical, electronic, and other industries. However, to date, there is still a lack of information regarding the human health and environmental implications of manufactured nanomaterials. Copper nanoparticles (nano-copper) have shown great promise as osteoporosis-treatment drugs, antibacterial materials, additives in livestock and poultry feed, and intrauterine contraceptive devices. Furthermore, nano-copper has been widely used in industry, e.g., as an additive in lubricants, for metallic coating, and as a highly reactive catalyst in organic hydrogen reactions. Usually, a variation in the size of metal nanoparticles results in bare nanoparticles possessing excessive surface energy, and this leads to an alteration in their catalytic properties. It is established that copper nanoparticles distribute in organs and tissues of animals and cause specific structural changes. The increase of copper nanoparticles in organism up to toxical threshold (maximum tolerated dose) results in dystrophy and tissue necrosis. Oxidative stress represents an imbalance between the production and manifestation of reactive oxygen species and a biological system's ability to readily detoxify the reactive intermediates or to repair the resulting damage. Disturbances in the normal redox state of tissues can cause toxic effects through the production of peroxides and free radicals that damage all components of the cell, including proteins, lipids, and DNA. Some reactive oxidative species can even act as messengers through a phenomenon called redox signaling. A particularly destructive aspect of oxidative stress is the production of reactive oxygen species, which include free radicals and peroxides. Some of the less reactive of these species (such as superoxide) can be converted by oxidoreduction reactions with transition metals or other redox cycling compounds (including quinones) into more aggressive radical species that can cause extensive cellular damage . The major portion of long term effects is inflicted by damage on DNA. Most of these oxygen-derived species are produced at a low level by normal aerobic metabolism and the damage they cause to cells is constantly repaired. However, under the severe levels of oxidative stress that cause necrosis, the damage causes ATP depletion, preventing controlled apoptotic death and causing the cell to simply fall apart .

Master Abstract PDF

PHD Title

Pulmonary and Pleural toxicity of Potassium octatitanate fibers, rutile titanium dioxidenanoparticles, and MWCNT-7 in male Fisher 344 rats

PHD Abstract

Pulmonary and pleural toxicity of potassium octatitanate fibers, rutile titanium dioxide nanoparticles, and MWCNT-7 in male Fischer 344 rats Because of its high tensile strength, chemical stability, and heat-resistance, POT is commonly used as an alternative to asbestos. POT fibers have a long needle-like shape, similar to asbestos, and are not broken down in the body. Thus, the fiber pathogenicity paradigm 2 identifies these fibers as potential carcinogens. The carcinogenicity of POT fibers was confirmed by an initial study that administered POT fibers by application of POT fibers directly to the pleural surface of Osborne-Mendel rats and a later study that administered POT fibers by intraperitoneal injection. Two recent studies infused POT fibers directly into the pleural cavity of mice and rats: one study followed the treated animals for 52 and 65 weeks and the other study followed the treated animals for 52 weeks. Although, none of the treated animals had developed mesothelioma by the time the study was terminated, all mice and rats treated with POT fibers exhibited pleural thickening. Inhalation studies that followed exposed animals for up to 2 years were mostly negative. Overall, these studies indicate that POT fibers are carcinogenic, but that a high amount of POT fibers in prolonged contact with susceptible tissue is required for induction of malignant neoplasia. They also suggest that inhalation of POT fibers is not carcinogenic in rats and that low level exposure may not be carcinogenic in humans. Thus, the physical characteristics of POT fibers suggest high carcinogenic potential, but the experimental evidence indicates low carcinogenic potential. A variety of factors could account for this discrepancy: (1) The fiber pathogenicity paradigm may not apply to POT fibers, possibly because of the chemical make-up of the fibers. (2) The relatively low sensitivity of inhalation studies using rats may be a factor in the negative results of the inhalation studies; the low sensitivity of rat inhalation studies is also noted. (3) If the fibers are not well dispersed, this will result in lower than expected levels of particles with small enough aerodynamic diameters to penetrate beyond the ciliated airways. Thus, the physical characteristics of POT fibers and the fact that they can induce malignant transformation in susceptible tissues suggest high carcinogenic potential, but inhalation studies indicate that respirable POT fibers have low carcinogenic potential in test animals. To investigate the possibility that this apparent discrepancy was due to the titanium dioxide composition of POT fibers, we conducted a short-term experiment to compare the toxicity of POT fibers with two titanium dioxide nanoparticles, anatase titanium dioxide nanoparticles (a-nTiO2) and rutile titanium dioxide nanoparticles (r-nTiO2). We administered POT fibers and a-nTiO2 and r nTiO2 to the lungs of rats using intra-tracheal intra-pulmonary spraying (TIPS) and found that POT fibers had greater biopersistence and induced a greater degree of toxicity in the lung than a-nTiO2 or r nTiO2. These results are in agreement with the findings of studies with other materials that fiber-shaped materials are more toxic to the lungs than spherical shaped nanoparticles of the same chemical composition. Thus, the titanium dioxide composition of POT fibers does not appear to explain their lack of carcinogenicity in the lung. Therefore, we are conducting a 2-year study comparing the lung toxicity of POT fibers to r-nTiO2 and MWCNT-7, a known lung carcinogen in rats. Overall, we found that at 1 year POT fibers had a similar or greater degree of toxicity in the lung and pleural cavity compared to MWCNT-7. At 1 year in rats administered POT fibers and MWCNT-7, there were persistent inflammatory and fibrotic changes in the lung and pleura, elevated alveolar macrophage counts, elevated levels of CCL2 and CCL3 in the lung tissue, increased levels of 8-OHdG adducts in the lung tissue DNA, and increased PCNA labeling of lung alveolar cells and visceral and parietal mesothelium. Hyperplasia of the visceral mesothelium was found in 2 of 5 rats in the 0.50 mg POT group and 1 of 5 rats in the 0.50 mg MWCNT-7 group. In contrast, none of these parameters was elevated in rats administered r-nTiO2. In conclusion, there is clear evidence that POT fibers are toxic in the lung and pleura of male rats. POT fibers were biopersistent in the lung and mesothelium of rats, provoking inflammation and tissue and DNA damage. These results are in agreement with the physical characteristics of these fibers and the potential adverse health effects of thin, long, biopersistent fibers. The pulmonary and pleural toxicity of POT fibers was similar or greater than that of MWCNT-7, a known carcinogen to the rat lung.

PHD Abstract PDF

Mohamed Ahmed Mahmoud Abdelgied

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Master Title

Master Abstract

PHD Title

PHD Abstract