Basic Informations

C.V

Ahmed O. Hassan
Education
Purdue University, West Lafayette, IN
PhD in Virology
2017
College of Veterinary Medicine, Beni Suef University, Egypt
Master’s in Veterinary Medical Sciences (Virology)
2011
Doctor of Veterinary Medicine (DVM); Ranked among top 10% in class
2005
Experiences and Positions
Postdoctoral Research Scientist (ORISE Fellow)
Merial Ltd., USA
2017- Current
o Developed a new generation of human adenovirus vector-based vaccines for FMD virus
o Established a system for testing the stability of the newly developed vaccines
Graduate Research Assistant
College of Veterinary Medicine, Purdue University, USA
2012 - 2017
o Generated human adenovirus vector-based multi-epitope vaccines for highly pathogenic H5, H7 and H9 avian influenza viruses
o Evaluated immunogenicity and protective efficacy of vaccine candidates in a mouse model
o Generated human and bovine adenovirus vector-based vaccines against emerging H7N9 avian influenza virus. Tested vaccine candidates in a mouse model
o Characterized a novel structural protein in bovine adenovirus 3 and tested the impact of this protein in developing a recombinant replication-defective bovine adenovirus vector as a vaccine platform
o Participated in different research projects conducted in the laboratory and with collaborators including molecular mechanisms for adenovirus packaging, dose sparing for adenovirus-based influenza vaccines, a novel nanoparticle adjuvant for seasonal influenza vaccines and longevity of adenovirus pre-existing immunity and its effect on efficacy of adenovirus-based influenza vaccines
o Served as lab manager for one year: ordering supplies, keeping record of lab reagents/plasmids/viruses, etc...
o Trained undergraduate and DVM summer research students in Virology techniques
o Taught some virology lectures to DVM students at Purdue University
Instructor of Virology
College of Veterinary Medicine, Beni Suef University, Egypt
2006 - 12
o Taught virology courses to DVM students
Research Assistant
College of Veterinary Medicine, Beni Suef University, Egypt
2006 - 12
o Master’s research project: isolation and molecular characterization of pestivirus isolates
o Participated in the research projects conducted in the Department of Virology including serological surveillance of different viruses from poultry and livestock, isolation and molecular characterization of different viral isolates from poultry and livestock, and development of vaccines against the local isolates and testing their efficacy.

Master Title

Studies on border disease virus in sheep.

Master Abstract

Seroprevalence of pestivirus infections in 40 sheep flocks within El-Minia and Beni-Suef Governorates was conducted using ELISA. All examined flocks were found to be positive for pestiviruses. Nine foeti and neonates from sheep and goats in Egypt were screened for pestiviruses using immunohistochemistry (IHC), virus isolation, and reverse transcription-polymerase chain reaction (RT-PCR). Two goat kids with typical border disease manifestations showed positive results with IHC using polyclonal anti-BDV serum but not with four different monoclonal antibodies (Mabs). On inoculation in MDBK, a cytopathic bovine viral diarrhea virus (BVDV) was isolated from one kid. Further sequencing of of 5'-UTR, Npro, and E2 genes revealed that the isolate was identified it as BVDV subtype 1b. Although the circulating virus in Egypt is considered to be BVDV 1a, this report confirms the existence of BVDV 1b in Egypt. Mutations were found in E2 genomic regions which might be the responsible for escaping of the virus for detection with Mabs that targets epitopes on E2 protein. To our knowledge, this is the first report of isolation of a pestivirus from goat in Egypt and is probably the second report worldwide of a goat kid showing central nervous signs associated with border disease.

PHD Title

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PHD Abstract

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