Basic Informations
C.V
Curriculum Vitae
Abdeltawab Abdelrazek
Yassin Khalil
Assistant Professor (Theriogenology)
Phone: +2 082 2322066
FAX: +2 082 2327982
E-mail:
yassyn999@yahoo.com; abdeltawab.yaseeen@vet.bsu.edu.eg
EDUCATION:
-1989
B.V. Sc. Veterinary Medicine, Cairo University/ Beni-Suef
-1995 M. V. Sc.
Veterinary Medicine, (Theriogenology) Cairo University/ Beni-Suef-
-2004 Ph.D., Veterinary
Medicine, (Theriogenology) Hannover / Germany
RESEARCH:
Dr Abdeltawab Abdelrazek
Yassin Khalil current research is on IFV and AI
TEACHING / INSTRUCTION:
Vet. Gynaecology
Vet. Obstetrics
Vet. Andrology
Artificial Insemination
In Vitro Fertilization
(IVF) & Embryo Transfer (ET)
CONFERENCES
Khalil AAY, Petrunkina AM, Sahin E, Waberski D,
Töpfer-Petersen E.(2006):Enhanced binding of sperm with superior volume
regulation to oviductal epithelium. J Androl. 2006 Nov-Dec;27(6):754-65. Epub
2006 Jun 28. PMID: 16809276 [PubMed - indexed for MEDLINE]
Khalil, A. A. Y.; Löhmer, I.;
Petrunkina, A. M.; Wrenzycki, C.; Waberski, D.: Binding capacity of bull
spermatozoa to oviductal epithelium in vitro and its relation to sperm
chromatin instability, sperm volume
regulation and fertility
In: Wiener tierärztliche Monatsschrift 91 (2 mit
Suppl. 2); 37. Jahrestagung über Physiologie und Pathologie der Fortpflanzung,
gleichzeitig 29. Veterinär- Humanmedizinische Gemeinschaftstagung,
19.-20.02.2004 München, GERMANY Public Relations, 2004, S. 38 ISSN
0043-535X
Waberski, D.; Khalil, A. Y. Y.; Ardon, A.; Magnus, F.; Helms,
D.; Wrenzycki, C.; Petrunkina, A. M.; Töpfer-Petersen, E.:
Sperm-oviduct binding in bull and boar and its relation to chromatin
stability, sperm volume regulation and fertility
In: Workshop proceedings; 15th International Congress on Animal
Reproduction, Porto Seguro, BRAZIL
8.-12.08.2004; S. 87
Waberski, D., Löhmer, I., Khalil, A. A. Y., Beyerbach, M.,
Töpfer-Petersen, E.:
Preferential binding of chromatin-stable bull spermatozoa to oviductal
epithelium in vitro, 7th conference of the European Society for
Domestic Animal Reproduction, Dublin, IRELAND,
4.-6.9.2003
In: Reproduction in domestic animals 38 (2003)
335-336
Waberski, D., Petrunkina, A. M., Friedrich, J., Magnus, F., Khalil,
A. A. Y., Töpfer-Petersen, E.:
In vitro-studies of sperm binding and survival at oviductal epithelium
in pigs and cattle, 7th conference of the European Society for
Domestic Animal Reproduction, Dublin, IRELAND ,
4.-6.9.2003
In: Reproduction in domestic animals 38 (2003) 325
List of publications
1. Abdeltawab Abdelrazek Yassin Khalil
(1995). Factors affecting fertility of frozen semen of cow and buffalo bulls
under field conditions. M. V. Sc. Thesis in Theriogenology, Cairo Univ.
Beni-Suef Branch, Fac. Vet. Med.
2. Yassin,
A. A.,
Hussein, M.M., Abdel-Gawad, E.M. and Gomaa, A. (1998): Factors affecting
conception rate in cattle. Egyptian Society of Animal Reproduction and
Fertility 10th Annual Congress, 3-5 Feb., 1998, Giza
/ Egypt.
3. Waberski, D., Löhmer, I., Khalil,
A. A. Y., Beyerbach, M., Töpfer-Petersen, E.: Preferential binding of
chromatin-stable bull spermatozoa to oviductal epithelium in vitro, 7th
conference of the European Society for Domestic Animal Reproduction, Dublin,
IRELAND, 4-6/9/2003. In: Reproduction in domestic animals 38 (2003) 335-336.
4. Waberski, D., Petrunkina,
A. M., Friedrich, J., Magnus, F., Khalil, A. A. Y., Töpfer-Petersen, E.:
In vitro-studies of sperm binding and survival at oviductal epithelium in pigs
and cattle, 7th conference of the European Society for Domestic
Animal Reproduction, Dublin, IRELAND, 4-6/9/2003. In: Reproduction in domestic
animals 38 (2003) 325.
5.
Khalil, A. A. Y.; Löhmer, I.; Petrunkina,
A. M.; Wrenzycki, C.; Waberski, D.: Binding capacity of bull spermatozoa to
oviductal epithelium in vitro and its relation to sperm chromatin instability,
sperm volume regulation and fertility. In: Wiener tierärztliche
Monatsschrift 91 (2 mit Suppl. 2); 37. Jahrestagung über Physiologie und
Pathologie der Fortpflanzung, gleichzeitig 29. Veterinär-Humanmedizinische
Gemeinschaftstagung, 19.-20.02.2004 München, GERMANY Public Relations, 2004, S.
38 ISSN 0043-535X.
6. Waberski, D.; Khalil, A.
Y. Y.; Ardon, A.; Magnus, F.; Helms, D.; Wrenzycki, C.; Petrunkina, A. M.;
Töpfer-Petersen, E.: Sperm-oviduct binding in bull and boar and its relation to
chromatin stability, sperm volume regulation and fertility. In: Workshop
proceedings; 15th International Congress on Animal Reproduction, Porto Seguro,
BRAZIL 8.-12.08.2004; S. 87.
7. Khalil
AAY, Petrunkina AM, Sahin E,
Waberski D, Töpfer-Petersen E. (2006): Enhanced binding of sperm with superior
volume regulation to oviductal epithelium. J Androl. 2006 Nov-Dec;27(6):754-65.
Epub 2006 Jun 28. PMID: 16809276 [PubMed - indexed for MEDLINE].
8. Waberski, D.; Petrunkina A.
M.; Khalil A. A. Y.; Töpfer-Petersen E. (2006): Functional significance
of sperm cell volume regulation for oviductal reservoir formation in cattle.
10th International Symposium on Spermatology programme, abstracts Madrid,
17.-22.09.2006; 2006, S. 103. http://www.spermadrid2006.org/archivos/File/Spermadrid/Spermadrid_Abstracts.pdf.
9. Abdel-Halim, B.R.,
Khalil, A.A.Y and Saadia, A. Ali (2008). Quality and Fertility of Bovine
Semen Cryopreserved in Egg-yolk Free and Egg-yolk Containing Extenders. 13th
Sci, Cong. 23- 25
November, 2008, pp517- 532, Fac. Vet. Med., Assiut Univ., Egypt.
10. Abd-Allah, S.M; A.A.Y.
Khalil and K.M. Ali (2008). The use of trypan blue staining to select the
developmentally component dromedary camel oocytes and its effect on in vitro
maturation rate. 20th Annual Scientific Conference, 2-7 February 2008, Egyptian
Society of Reproduction and Fertility, Fayoum, 207-218.
11. Hussein,M.M. ; El Agawany
,A. A and AAY Khalil (2009). Effect of dietary cation-anion difference
(DCAD) on plasma calcium level, urine ph, pattern of parturition and post
calving related disorders in Holstein dairy cows. Alex. J. Vet. , Med. Vol. 28,
No. 1, March 2009 (455-475). ISSN 110-2047 ALEX. J. VET. SCIENCE 2009 VOL 28 NO. 1(5th SCVMR).
12. Hussein, M.M; A.A El
Agawany and A.A. Khalil (2009). Influence
of subclinical mastitis on some reproductive parameters in Holstein-Friesian
dairy cows. Vet.
Med. J. Giza. Vol. 57, No. 1. 97-113.
13. Abdel-Halim, B.R. and A.
A. Y. Khalil, (2009). Fetal sex determination in buffalo cows (bubalus
bubalis) by using ultrasonography. Egypt. J. Basic Appl. Physiol. 8 (1): 175-184 (2009).
14. Ali, Saadia A; Khalil,
A. A. Y and Helmy, Nermeen A (2009). Effect of bovine ovary
cryopreservation on oocyte recovery and in vitro maturation. Egypt. J. Basic
Appl. Physiol. 8 (2): 477-486
15. Hussien, M. M.;
Abdel-Fattah, M.; Khalil, A. A.Y. and Sedeek, A. M. (2009). Evaluation
of a modified surgical technique for correction of vaginal and uterine prolapse
in bovine. Vet. Med.J., Giza Vol. 57, No. 1. (2009): 115-131.
16. Abdel Mohsen M. Hammam; El-
Sayed M.M. Abdel Gawad; Abdel Tawab A. Yassein; Mona S. Zaki; Amal H.
Ali (2012). Effect of PGF2α Double Injection on Hematological Picture, Hormonal
Levels and Fertility of Cows During Summer Season. Life Science Journal 2012;9
(4): 2356- 2362.
17. Khalil,
A.A.Y.
(2015). Factors affecting treatment response to Ovsynch protocol in Holstein
dairy cows suffered from cystic ovarian disease. Assiut Vet. Med. J. Vol. 61
No. 144 January 2015.
18. Hussein
M M; Khalil A.A.Y; Al Agawany A A and A A Zyada (2017): Efficacy of Ultra-sonographic
Examination and Combined Use of PGf2α plus Cephapirin to Identify and Treat
Endometritis in Dairy Cows. Journal
of Veterinary medical research 2017, 24 ( 2 ): 322-336.
19. Khalil A.A.Y, Hussein M.M.,
EL-Agawany A.A. and A.A. Zyada (2017): Impact of systemic PGF2α plus
intrauterine benzathine cephaprine combination in treatment of clinically and
ultrasonographically diagnosed endometritis in Holstein dairy cows. Assiut Vet.
Med. J. Vol. 63 No. 154: 153-163.
20. Abdel
Aziz, R.L., A.A.Y. Khalil, A. Abdel-Wahab, Nour Ell-Houda Yassein, E.
Abdel-Hamied and R. K. Kasimanickam (2017): Relationship among circulating
anti-Mullerian hormone, insulin-like growth factor 1, Cadmium and
superovulatory response in dairy cows. Theriogenology 100: 72-79.
21. Abdel
Aziz, R. L., A. Abdel-Wahab, Fatma I. Abo El-Ela, Nour El-Houda Y. Hassan,
El-Shaymaa El-Nahass and Marwa A. Ibrahim and A. A. Y. Khalil (2018):
Dose dependent ameliorative effects of Quercetin and L-Carnitine against
Atrazine-induced reproductive toxicity in adult male Albino rats. Biomedicine
and Pharmacotherapy 102: 855-864.
22. Abdeltawab
Abdelrazek Yassin Khalil (2019) Fertility response of lactating dairy cows subjected to three
different breeding programs under subtropical conditions. Khalil Beni-Suef
University Journal of Basic and Applied Sciences (2019) 8:6 https://doi.org/10.1186/s43088-019-0008-x.
23. Abdel-Tawab
A. Y. Khalil,
Ahmed Abdel-Wahab, Rabie L. Abdel Aziz (2019) Relationship between heart girth,
serum progesterone and superovulation response of donor Holstein cows. Asian
Pacific Journal of Reproduction 2019; 8(3): 118-123.
24. Abdel-Tawab
Abdel-Razek Yassyn Khalil, Mahmoud Mohamed Hussein (2019): Pregnancy outcome of lactating dairy cows
assigned for Presynch-Ovsynch synchronization program and inseminated either at
detected standing heat or at scheduled fixed time. Asian Pacific Journal of
Reproduction 2019; 8(4): 181-186.
25. Abdeltawab
Y. Khalil
(2019): Comparative Efficacy of Two Synchronization of Ovulation Regimens in
The Treatment of Cystic Ovarian Disease in Dairy Cows. Alexandria Journal of
Veterinary Sciences. Vol. 62(1): 61-71 July 2019
26. Abdeltawab
A.Y. Khalil
and Mahmoud M. Hussein (2019) A retrospective study on the relationship among
different dry period lengths, udder health status and their possible effects on
the reproductive performance of Holstein-Frisian cows (2019). Journal of
Veterinary Medical Research 26 (2): xx-xx. In Press
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Master Title
Factors Affecting Fertility of Frozen Semen of Cow and Buffalo Bulls Under Field Conditions
Master Abstract
Summary and Conclusion
During the last few decades attempts have been made to introduce the freezing technique in A.I. centers. The reasons for that are obvious and it is believed that by the use of frozen semen the genetically superior bulls will be used fully and improvement in production will be realized at a shorter period. It seems necessary therefore to evaluate the introduction of such practice under the Egyptian conditions of management. However, the fertility results are not only dependent on the fertility of semen but also on the fertility potentials of the recipient animals. Therefore, it seems essentials to evaluate the fertility results of both cattle and buffaloes after utilization of deep-frozen semen and study the factors affecting such results under field conditions. The present study is a modest attempt in this direction.
The current study was conducted in four villages in Beni-Suef Governorate. 2021 animals were included and categorized into 590 Baladi cows, 198 Baladi heifers, 633 crossbred cows, 245 crossbred heifers, 259 Buffalo cows and 96 Buffalo heifers. These animals were owned sporadically by farmers. Gynaecological examination of all animals was performed and only the healthy ones were inseminated by frozen semen which obtained from Beni-Suef artificial insemination canter, Abbasia Buffalo frozen semen production unit, or imported from France. This study was carried out from January 1994 to April 1995. Pregnancy was diagnosed by rectal palpation 60-90 days following insemination day. Records including the name and address of the owner, breed, age, parity, postpartum service interval, condition of the last parturition and nature of the cervical mucus and insemination date. The bull number, date of semen processing and thawing regime were also included in these records.
The data were subjected to statistical analysis by ANOVA test and least square means (LSM) using the statistical SAS software (1995).
The results of the present study revealed the following:
• About 44.9% of cattle and 23.8% of buffaloes were utilized artificial insemination in the area under study.
• The overall mean conception rate (CR) in heifers was higher (55.84±1.74) than that recorded in cows (49.62±1.0). nearly the same trend was observed in Buffaloes, as the CR that recorded in B-heifers was (47.3±2.04) higher than that recorded for B-cows (44.23±2.41).
• Both cattle and buffalo heifers required less number of services per conception (NSPC) than pleuriparous cows.
• The seasonal variations exerted a significant effect on the CR in both cattle and buffaloes, where the highest CR were obtained during Autumn (56.09±1.04 &54.11±5.23) and winter (55.65±1.04 & 53.33±43.37) for cows and buffaloes respectively. The lowest CR were obtained during summer (39.76±2.38 & 32.64±6.65) and Spring (47.0±2.08 & 36.84±6.29) for cows and buffaloes respectively. However, the fertility results of heifers were not affected by season of insemination
• Animals bred during Summer and Spring required more number of inseminations to become pregnant than those bred during Autumn and Winter.
• The time elapsed from estrus detection till insemination exerted a highly significant effect on the CR in both cattle and buffaloes, and the higher CR were recorded in cows (65.76±1.26) and buffaloes-cows (54,65±3.23) when inseminations were carried out at 12-18 hours after the first estrus signs was detected, while the highest CR were recorded in heifers (69.74±3.72) and buffalo-heifers (61.85±3.65) when inseminations were done before 12 hours from first estrus detection.
• Generally, animals inseminated after 18 hours from estrus detection required more inseminations per one conception.
• The nature of the cervical mucus at the time of insemination exerted a highly significant effect on the CR in both cattle and buffaloes, where the highest CR was obtained in cows (70.35±1.27), heifers (75.06±2.31), buffalo-cows (53.65±4.92) and in buffalo-heifers (60.72±4.71) when inseminations were performed in the presence of clear and glassy cervical mucus, while inseminations that done in the presence of thin and watery cervical secretions resulted in significant lower CR (22.23±2.62, 31.13±5.72, 26.14±7.08 and 23.5±8.16) in cows, heifers, buffalo-cows and buffalo-heifers respectively.
• Animals with thin and watery cervical secretions required significant a greater number of inseminations to become pregnant than animals with clear and glassy mucus, and those with scanty mucus at the time of inseminations.
• Regarding the effect of parity, the highest CR were recorded in cows (62.95±1.31) and buffaloes (52.85±3.36) having 2-4 calvings, while primiparous and multiparous animals having more than 4 calvings the CR were decreased and the NSPC were increased.
• Concerning the postpartum service intervals, the highest CR were obtained in cows (66.64±1.33) and buffaloes (47.36±3.78) and lower NSPC in cows (1.5±0.01) and buffaloes (2.1±0.05) when inseminations were done at 45-90 days postpartum.
• The condition of the last parturition exerted a highly significant effect on the fertility in cattle and buffalo, where the highest CR were recorded for cows (60.89±1.09) and buffaloes (52.47±5.78) and the lowest NSPC for cows (1.64±0.01) and buffaloes (1.9+0.03) that calved normally.
• The differences in CR an NSPC due to the effect of breed/genotype of cattle, semen source, service sire, and sequence of insemination were not significant.
• A highly significant negative correlation coefficients between CR and the percentage of sperm head abnormalities (-0.87), total sperm abnormalities (-.79), percentage of abnormal acrosome (-0.77), % of proximal protoplasmic droplets (-0.77) and % of detached sperm heads 9-0.71). However, a significant positive correlations were obtained between the CR and live sperm % (+0.65), post hawing progressive forward sperm motility % (+0.56).
Conclusions
From the results of the current study and according to the used fertility measures we can conclude that:
1) -No significant differences in fertility results were noticed between Baladi cows and their crosses.
2) -No significant differences in fertility results were obtained between locally prepared and imported frozen bovine semen.
3) -The Winter and Autumn are the best breeding seasons.
4) -Heifers were less affected by seasonal variations.
5) -The best time to inseminate multiparous cows was 12-18 hours after estrus detection and was found to be < 12 hours post estrus detection to inseminate heifers.
6) -The fertility of heifers was higher, decreased after the first calving, then increased till reach maximum at 2-4 calvings, then gradually decreased.
7) -Dystocia, abortion, still birth, retained fetal membranes, vaginal and uterine prolapse were greatly negatively affect the subsequent fertility.
8) -Bulls recorded high fertility measures in the lab, recorded also t a great extent high fertility results in the field (in vivo).
PHD Title
BINDING CAPACITY OF BULL SPERMATOZOA TO OVIDUCTAL EPITHELIUM IN VITRO AND ITS RELATION TO SPERM CHROMATIN STABILITY, SPERM VOLUME REGULATION AND FERTILITY
PHD Abstract