Basic Informations

C.V

Nashwa Shehata Abdelhady Shabaan

Address: Bani Suef- Ahmed Ramy st, villa 90        

Phone: 01100632177

E-mail: Nashwa.Shehata@vet.bsu.edu.eg

Date of birth: 07-04-1994

Nationality: Egyptian

National ID: 29404078800286

Education:

-B.V. Sc.2017 Fac. Vet. Med-beni-suef University.

- M.V.Sc 2023 in Animal and Poultry behaviour and management.

Master Title

Effect of some medicinal plants on behaviour and performance of sheep at different environmental conditions

Master Abstract

Improvement of sheep health and productivity became a global demand. A convenient house has to provide adequate space, sheltering, and protection from climatic changes and stress. Some plants such as thyme (Thymus vulgaris), celery (Apium graveolens) and curcumin could alleviate stressors. Hence, this study was investigated to determine the role of thyme and celery seed mixture (TCM) as well as curcumin in improving the behavior and performance of sheep at different housing conditions during autumn and winter. Experiment 1: Effect of thyme and celery mixture (TCM) on behaviour and performance of pregnant and lactating ewes under different housing conditions Forty pregnant ewes (2 years old and 48±1.5 kg average body weight) were randomly equally distributed into two buildings (20 for each). In Semi shaded building; SSB (roofed with a concrete slab 40 cm in thickness and 5 meters in height, and covered with three rows of rice straw bales as thermal insulation and a natural dirt land towards the north and south.), 10 ewes fed on basal diet and the others were received a basal diet containing Thyme and celery mixture (TCM) (10 gm thyme and 10 gm of celery/head/day). Similarly, 20 ewes were treated in the full-shaded building; FSB (roofed with a layer of asbestos, 5 meters in height, and a natural floor in the west and east). Ewes start fed on basal diet +TCM for one-month pre-lambing and two months post-lambing. Feeding both behavior and weight were bi-weekly recorded. Blood samples were collected each month for measuring oxidative stress indicator reduced glutathione (GSH), malondladhydes (MDA), total protein, glucose. In addition, gene expression of Nuclear factor 2 linked to erythroid 2 (Nrf2), and interleukin 2 at the first, second and third month of lactation. Cumulative feed intake was bi-weekly calculated. Milk samples were collected one month after lambing till weaning to measure moisture, ash, total protein, lactose, non-solid fat (NSF), total solids, fat, and antioxidant capacity of milk (free radical and inhibition factor). As a result full shaded houses succeed to protect pregnant ewes from environmental changes despite the decreased feed intake. Thyme and celery mixture (TCM) supplementation didn’t achieve success in mitigating the physiological stress of pregnancy in both housing conditions but resulted in a significant increase in the feed intake of semi-shaded housed ewes. Moreover, the findings of lactating ewes revealed that sheep exhibited better feeding behavior in FSB and the oxidative stress indicator was less than that of SSB. Thyme and celery mixture (TCM) enhanced feeding behavior and decreased oxidative stress indicator (reduced GSH) in SSB. In addition, it increased total protein and dam body weight in FSB and improved FI in both buildings. Thyme and celery have antioxidant capacity in milk. Hence, the present data suggested that TCM addition to the basal diet could reduce the stress of climatic changes on lactating ewes. Second experiment "Effect of curcumin on behaviour and performance of lambs at three different housing conditions" This experiment aimed to investigate effect of use curcumin (Cu) on temperament, performance and fertility of lambs under three different housing conditions. Lambs (6 months average of age and average body weight; 22±1.5 kg) were randomly allocated into three houses (semi-shaded building; SSB, fully-shaded building; FSB and semi-shaded building with trees SSB+T) 1 g curcumin was added freshly to each lamb daily of 3 months. Twelve lamb in each house that divided equally into non-treated (control; n=6) and treated (Cu; n=6) groups. Every month, Temperament test was scored and blood was collected for measuring of glutathione, malondaldhyds, total protein, glucose and genes analysis: myostatin and insulin growth factor 1 (IGF-1). Every 2 weeks feed intake and body weight gain was calaulated, testesteron level was measured at the last month. As a result, temperemnt was non-significantly disturbed in SSB while, FI is increased in SSB. Lambs, body gain were increase in FSB and SSB+T at 8th. Glucose level was increased at first 2 months then decreased at the 3rd month in SSB+T. Though malondaldhyds level was not affected at different houses, GSH activity was increased in lambs kept in SSB+T. Testosterone levels decreased significantly in the SSB in comparison with FSB and SSB+T. In addition, curcumin improved lambs, temper and feed intake in all houses. Moreover, curcumin increased glucose, and testosterone in lambs reared in SSB while, it reduced total protein (in FSB) and caused oxidative stress in FSB and SSB+T at the 3rd month. In addition, it decreased testosterone level in SSB+T at the 3rd month. Mean awhile, it had no significant effect on body gain lambs throughout the experiment. Data suggested curcumin could not improve lambs growth while it improved animal temper and feeding in all houses and ameliorated the recorded reduction in fertility in the semi-shaded house which is commonly used in sheep.

PHD Title

PHD Abstract

Improvement of sheep health and productivity became a global demand. A convenient house has to provide adequate space, sheltering, and protection from climatic changes and stress. Some plants such as thyme (Thymus vulgaris), celery (Apium graveolens) and curcumin could alleviate stressors. Hence, this study was investigated to determine the role of thyme and celery seed mixture (TCM) as well as curcumin in improving the behavior and performance of sheep at different housing conditions during autumn and winter. Experiment 1: Effect of thyme and celery mixture (TCM) on behaviour and performance of pregnant and lactating ewes under different housing conditions Forty pregnant ewes (2 years old and 48±1.5 kg average body weight) were randomly equally distributed into two buildings (20 for each). In Semi shaded building; SSB (roofed with a concrete slab 40 cm in thickness and 5 meters in height, and covered with three rows of rice straw bales as thermal insulation and a natural dirt land towards the north and south.), 10 ewes fed on basal diet and the others were received a basal diet containing Thyme and celery mixture (TCM) (10 gm thyme and 10 gm of celery/head/day). Similarly, 20 ewes were treated in the full-shaded building; FSB (roofed with a layer of asbestos, 5 meters in height, and a natural floor in the west and east). Ewes start fed on basal diet +TCM for one-month pre-lambing and two months post-lambing. Feeding both behavior and weight were bi-weekly recorded. Blood samples were collected each month for measuring oxidative stress indicator reduced glutathione (GSH), malondladhydes (MDA), total protein, glucose. In addition, gene expression of Nuclear factor 2 linked to erythroid 2 (Nrf2), and interleukin 2 at the first, second and third month of lactation. Cumulative feed intake was bi-weekly calculated. Milk samples were collected one month after lambing till weaning to measure moisture, ash, total protein, lactose, non-solid fat (NSF), total solids, fat, and antioxidant capacity of milk (free radical and inhibition factor). As a result full shaded houses succeed to protect pregnant ewes from environmental changes despite the decreased feed intake. Thyme and celery mixture (TCM) supplementation didn’t achieve success in mitigating the physiological stress of pregnancy in both housing conditions but resulted in a significant increase in the feed intake of semi-shaded housed ewes. Moreover, the findings of lactating ewes revealed that sheep exhibited better feeding behavior in FSB and the oxidative stress indicator was less than that of SSB. Thyme and celery mixture (TCM) enhanced feeding behavior and decreased oxidative stress indicator (reduced GSH) in SSB. In addition, it increased total protein and dam body weight in FSB and improved FI in both buildings. Thyme and celery have antioxidant capacity in milk. Hence, the present data suggested that TCM addition to the basal diet could reduce the stress of climatic changes on lactating ewes. Second experiment "Effect of curcumin on behaviour and performance of lambs at three different housing conditions" This experiment aimed to investigate effect of use curcumin (Cu) on temperament, performance and fertility of lambs under three different housing conditions. Lambs (6 months average of age and average body weight; 22±1.5 kg) were randomly allocated into three houses (semi-shaded building; SSB, fully-shaded building; FSB and semi-shaded building with trees SSB+T) 1 g curcumin was added freshly to each lamb daily of 3 months. Twelve lamb in each house that divided equally into non-treated (control; n=6) and treated (Cu; n=6) groups. Every month, Temperament test was scored and blood was collected for measuring of glutathione, malondaldhyds, total protein, glucose and genes analysis: myostatin and insulin growth factor 1 (IGF-1). Every 2 weeks feed intake and body weight gain was calaulated, testesteron level was measured at the last month. As a result, temperemnt was non-significantly disturbed in SSB while, FI is increased in SSB. Lambs, body gain were increase in FSB and SSB+T at 8th. Glucose level was increased at first 2 months then decreased at the 3rd month in SSB+T. Though malondaldhyds level was not affected at different houses, GSH activity was increased in lambs kept in SSB+T. Testosterone levels decreased significantly in the SSB in comparison with FSB and SSB+T. In addition, curcumin improved lambs, temper and feed intake in all houses. Moreover, curcumin increased glucose, and testosterone in lambs reared in SSB while, it reduced total protein (in FSB) and caused oxidative stress in FSB and SSB+T at the 3rd month. In addition, it decreased testosterone level in SSB+T at the 3rd month. Mean awhile, it had no significant effect on body gain lambs throughout the experiment. Data suggested curcumin could not improve lambs growth while it improved animal temper and feeding in all houses and ameliorated the recorded reduction in fertility in the semi-shaded house which is commonly used in sheep.