Basic Informations

C.V

Name:           Eman Saeed Ahmed

Job:              demonstrator at faculty of nursing Beni-Suef University

Mobile:            01122807866              

E-mail:              emansaied043@gmail.com    

Address:           Beni-suef

Nationality:    Egyptian

Birth Date:     2/8/1994

Marital Status: single

Religion:            Muslim

Languages:

*    Arabic:    Native Language

*    English:  Very Good

Computer skills: -

*    Very Good Knowledge of (Windows XP, Word, Power Point, Internet)

*    Good Knowledge of (Excel, Access)

*    Information technology IT   

Personnel skills: -                                                   

Good time-management and organizational skills. 

Hard working.

I can develop myself to be fit for any responsibility 

Able to work as a part of teamwork

Work under stress.

Lecturing in English language.

Presentation skills.

Creativity and development.

Communication skills

Main Objective;

1)    Development of my scientific and practical Skills in academic field.

2)    Acquiring more Work Experiences in my field as academic lecturer.

Education: -

ü Graduated from the Faculty of Nursing, Beni-Suef University. An excellent degree with honors in 2017.

ü Graduated from Health Technical institute of nursing in 2014.

ü TOEFL English course with 460 score in 2022.

ü Digital transformation course with an excellent degree in 2023.

Courses and workshops.

*    Participated in the training workshop on Pathophysiology of coronavirus 31st March 2021

*    Participated in the training workshop on technology of using interactive electronic wall padlet 28 March 2021

*    Participated in the training workshop on A guide to effective presentation at academic conferences 10th April 2021

*    Participated in the training workshop on scientific manuscript formatting using MS word 27th March 2021

*    Attendance in scientific workshop in the quality unit entitled "Evaluation of the examination Paper" on 13, December, 2021.

*    participated in scientific workshop of community health nursing department entitled " Sustainable development and enhancing the role of community health nursing in health and disease" " with talk of " puerperal sepsis" on 23th December, 2020

*    participated in scientific workshop of community health nursing department entitled " Sustainable development and enhancing the role of community health nursing in health and disease" " with talk of " bronchitis in children under 5 years" on 23th December, 2020

*    Contribution as attendance in scientific workshop entitled " Sustainable development and enhancing the role of community health nursing in health and disease" on 23th December, 2020

*    Attendance in scientific workshop entitled "??????? ?????? ?????? ???????? ??????    ??????? ????? ?????? ?????????" at faculty of nursing, Beni-Suef University.

An educational seminar on diabetes and how to prevent diabetic foot for students and teachers at Al Shorouk Experimental School

An educational seminar on smoking, its harm, and how to stop this bad habit

Holding a course on first aid course and training procedure which include:

Bandages and dressings

Basic life support

Poisoning                                         -

Chocking                                           

Vital Signs

Injection

Heart and lung resuscitation

Burns

Fractions

Snake bite

Swallowing the tongue

Bleeding of all kinds

An educational seminar on rheumatic fever for students at Al Shorouk School

An educational seminar on diseases common to students at the school stage, such as mumps, anemia, measles, diabetes........

An educational seminar on virus A,B, C and how to prevent them  at Beni Suef University Hospital

An educational seminar on dengue fever for employees and employees of the Faculty of Nursing, Beni Suef University.

An educational seminar on disability for employees and workers of the Faculty of Nursing, Beni Suef University.

Attendance in scientific workshop entitled “E-courses” Development and quality unit.      ( 30 December 2016)

Contribution as attendance in scientific workshop entitled “Exam Preparation” which includes Write Questionnaire and calculation.                                               (  13 December 2016 )

Attendance and positive participation in workshop entitled “Heart between science and faith”.  ( 27 October 2016)

Contribution as attendance in scientific workshop entitled “Exam Evaluation”                            ( 18 October 2016 )

Attendance and positive participation in workshop entitled “Natural reserves in Egypt; Treasures of an ancient”.   

(1 November 2016)

Research methodology course      (1 August 2016).

Management of laboratories course (15 March 2015).

Work Experience:-

Internship year at 57357 cche from 10/2017 to 3/2019.

Demonstrator at the Faculty of Nursing Department of Community Health, Beni Suef University, for 4 years.

Work as a nurse in ICU unit in Beni Suef general hospital from 6/2015 to 5/2016.

A training course on Nasser Institute Hospital in 2015.

A training course on the academic decisions report.

ICDL Course

TOEFL course in 2022.

Conferences.

Positive participation in organizing the scientific workshop of the Community Health Nursing Department entitled "Sustainable Development and Strengthening the Role of Community Health Nursing in Health and Disease" on Wednesday, 23rd December 2020.

Positive participation in the scientific workshop of the Community Health Nursing Department entitled "Sustainable Development and Strengthening the Role of Community Health Nursing in Health and Disease" with a scientific presentation titled "Bronchitis in children "and" puerperal sepsis " on Wednesday, 23rd December 2020.

Participating in a workshop for faculty members and their assistants on the examination paper and correction criteria in light of the quality requirements of the Faculty of Nursing, Beni Suef University

Organizing and participating in organizing the Scientific Day of the Community Health Nursing Department on Women's Health

Active participation and attendance at a workshop entitled How to prepare the course file.

Active participation in the third student conference of the Community Health Department under the title (Community Health Nursing and Mother's Health)

Active participation in the twelfth scientific conference of the pediatric department of the College of Human Medicine under the title (Causes and Complications of Anemia).

Participation in organizing the scientific day for the Department of Community Health Nursing

Active participation in the third scientific student conference under the title (Scientific research is our way to advance the profession of nursing.

Attendance and positive participation in the scientific conference of the Department of Community Health at Beni Suef University entitled (Community and Occupational Health Nursing and Disability).

Active participation in the activities of the conference  8th Beni- Suef university faculty of nursing conference ( Nursing development by integrating with related sciences. 15-17 april 2018 Beni-Suef University- conference center hotel- Ain Sokhna)

Active participation in the implementation of the educational and training convoy in the village of Sades Beni Suef, which aims to raise health awareness for the community

Active participation and attendance at a workshop on course description in light of quality and accreditation requirements

Passing a training course in the field of communication skills and professional ethics.

Passing the training course in infection control principles

Attendance Conference entitled “Student To Researcher”   (27 December 2016)

Attendance the 1st International Nursing Conference faculty of nursing beni-suef University.          (26 April 2016)

Attendance the 1ST Annual Student’s Conference of the Faculty of Nursing Beni-Suef University Entitled   “Quality In Nursing: A Holistic View& Future Vision”                  (19th March, 2015 )

Participation as a member in organizing committee in the 1st annual Student’s Conference of Faculty of Nursing, Beni-suef University Entitled  “ Quality in Nursing :A Holistic View & Future Vision “    on    (19th March, 2015 ).

Certificates of Appreciation and Honor.

*    Appreciation for my Participation in the advancement of the educational process in my department “community Health Nursing Department” Beni Suef University. (5th March, 2019).

*    Appreciation for my positive Participation in the preparation of Manual Procedures Book for academic year of 2019/2022.

*    Appreciation for my Participation in Haptic optical reproduction technology is a new path for the future teacher 17 March 2021 

Thanks a lot for handling my CV

Master Title

Bacterial pathogens associated with mastitis and endometritis in sheep and goats

Master Abstract

The results of clinical examination of the udders of lactating ewes (n=90; 180 udder halves) and does (n=32; 64 udder halves) revealed that out of 244 examined udder haves, 158 halves were apparently normal;115 in ewes and 43 in she-goats, while 86 halves showed complete loss of function;58 in ewes and 28 in she-goats. A total number of 125 bacterial isolates were recovered from 122 milk samples from sheep and goats including 73 (58.4 %) Gram negative and 52 (41.6 %) Gram positive bacterial isolates. Gram negative bacterial isolates (n=73) were identified as 68 E. coli (54.4 %), 3P. multocida (2.4%) and 2M. haemolytica (1.6%). On the other hand, Gram positive bacterial isolates (n=52) as 20 Streptococci (16% including S. agalactiae 13 (10.4%), S. dysgalactiae 4 (3.2%) and S. uberis 3 (2.4%) and 32 S. aureus (25.6 %). Regarding animals, results of endometritis followed by collection of uterine swabs from sheep and goats showed that out of 84 apparently healthy examined animals, a total of 80 animals (68.97%) were positive (subclinical endometritis), while 4 animals (3.44%) were negative isolates from the samples of endometritis (116 samples). A total number of 113 bacterial isolates were recovered from 116 examined sheep and goats and identified as follow; 89 (78.76 %) Gram negative and 24 (21.23 %) Gram positive bacterial isolates. Gram negative bacterial isolates (n=89) were identified as 69 E. coli (61.06 %), 4 P. multocida (3.5%), 13 P. aeuginosa (11.5%) and 3M. haemolytica (2.65%). On the other hand, Gram positive bacterial isolates (n=24) as 6 E. faecalis (5.3 %), 5 E. faecium (4.44%), 4 Streptococci (3.53 %) and 9 S. aureus (7.96%). The results of haemolytic activity of bacterial isolates (selected representatively) from mastitis milk samples on sheep blood agar revealed that 70.5% of the bacterial isolates were haemolytic; 47.6% were ß-haemolytic while 22.9 % were a-haemolytic. Haemolysis was represented as 100%, 90%, 85.71% and 56% in M. haemolytica, Streptococcus spp., Staphylococcus spp. and E. coli, respectively. They were mostly ß-haemolytic 29 isolates (47.6%) while, 18 isolates (29.5%) were ?-haemolysis. The results of haemolytic activity of bacterial isolates from endometritis samples (selected representatively) on sheep blood agar of different bacterial pathogens revealed that 56.8% of the bacterial isolates were haemolytic; 20.3% were a-haemolytic and 36.5% were ß-haemolytic. Haemolysis was represented as 100%, 87.5%, 85%, 61.1% and 40% in M. haemolytica, Streptococcus spp., Staphylococus spp., E. coli and P. aeruginosa, respectively. In addition, 27 isolates were ß -haemolytic with a percentage of 36.5%. Whereas, 32 (43.2%) of the isolates were ?-haemolysis. Biofilm production was represented as 39 E. coli isolates (65%) (18 isolates from mastitis 30% and 21isolates from endometritis 35%), 20 Staphylococci isolates (66.67%) (16 isolates from mastitis 53.33% and 4 isolates from endometritis 13.34%), 7 Streptococci isolates (70%) (5 isolates from mastitis 40%) and 3solates from endometritis (30%), 3P. aeruginosa isolates (42.85%), 3 P. multocida (50%) (1 isolates from mastitis 16.67% and 2 isolates from endometritis 33.33%) and two isolates from endometritis 66.67% and 2 Enterococci isolates (one isolate of E. fecalis from goat and one isolate from E. faecium from sheep) (40%) from uterine samples. The results of in-vitro antimicrobial susceptibility of mastitis milk samples against 17 antimicrobial agents showed high susceptibility against ofloxacin (76%), followed by co-trimethoprim (73.6%), sulfamethoxazole-trimethoprim (71.2%), ciprofloxacin (70.4%), fosfomycin (68.8%), chloramphenicol (67.2 %) and doxycycline (65.6%). On the contrary, most of the isolates were resistant to amoxicillin-clavulanic acid (75.2 %), cefotaxime (72.8 %), amoxicillin (69.6 %), cephalexine (68.8 %), and cefotriaxone (64.8%). All bacterial isolates recovered from uterine samples were tested for in-vitro antimicrobial susceptibility against 17 antimicrobial agents of 9 different antimicrobial classes. The results showed high susceptibility against co-trimethoprim (76.11%) followed by ofloxacin (73.45%), fosfomycin (72.57%), doxycycline (71.68 %), ciprofloxacin (69.91%), sulfamethoxazole-trimethoprim (69.03%), tetracycline (69.02%) and chloramphenicol (67.26%). On the contrary, most of the isolates were resistant to amoxicillin-clavulanic acid (76.11%), cefotaxime (71.68 %), amoxicillin (70.79 %), cephalexine (69.02 %) and cefotriaxone (58.41 %). Using PCR to assess biofilm associated genes and MDR genes in different bacterial isolates as E. coli isolates harbored blaTEM genes (100%), while, qnrA gene was detected in 50% of the tested isolates of ovine endometritis. Meanwhile, all tested isolates harbored fimH virulence genes (100%). The iutA was detected in 25% of the tested isolates of endometritis of sheep only. P. aeruginosa harbored blaTEM, tetA, toxA and las1 with a prevalence rate of 100%. S. aureus isolates harbored mecA and coa (100%). The tetK and icaA genes were detected in 75% of the tested isolates. Streptococci isolates harbored aac(6')aph(2'') gene (100%). The tetO, cylE and cfb were detected in 75% of the tested isolates. P. multocida isolates isolates harbored nanB, toxA and omp87 with a prevalence rate of 100%. M. haemolytica isolates harbored gcp, itkC and ssa with a prevalence rate of 75%. The results concluded that there is a strong correlation between the phenotypes and genotypes of AMR in different bacteria. Moreover, biofilm is a main problem for dairy farms affecting the udder health, uterine tissue and is considered as a risk for milk quality, fertility and consumer health. Also, biofilm formation, in turn, is associated with acquiring MDR by a high prevalence of bacterial pathogens recovered in this study. .

PHD Title

Bacterial pathogens associated with mastitis and endometritis in sheep and goats

PHD Abstract

The results of clinical examination of the udders of lactating ewes (n=90; 180 udder halves) and does (n=32; 64 udder halves) revealed that out of 244 examined udder haves, 158 halves were apparently normal;115 in ewes and 43 in she-goats, while 86 halves showed complete loss of function;58 in ewes and 28 in she-goats. A total number of 125 bacterial isolates were recovered from 122 milk samples from sheep and goats including 73 (58.4 %) Gram negative and 52 (41.6 %) Gram positive bacterial isolates. Gram negative bacterial isolates (n=73) were identified as 68 E. coli (54.4 %), 3P. multocida (2.4%) and 2M. haemolytica (1.6%). On the other hand, Gram positive bacterial isolates (n=52) as 20 Streptococci (16% including S. agalactiae 13 (10.4%), S. dysgalactiae 4 (3.2%) and S. uberis 3 (2.4%) and 32 S. aureus (25.6 %). Regarding animals, results of endometritis followed by collection of uterine swabs from sheep and goats showed that out of 84 apparently healthy examined animals, a total of 80 animals (68.97%) were positive (subclinical endometritis), while 4 animals (3.44%) were negative isolates from the samples of endometritis (116 samples). A total number of 113 bacterial isolates were recovered from 116 examined sheep and goats and identified as follow; 89 (78.76 %) Gram negative and 24 (21.23 %) Gram positive bacterial isolates. Gram negative bacterial isolates (n=89) were identified as 69 E. coli (61.06 %), 4 P. multocida (3.5%), 13 P. aeuginosa (11.5%) and 3M. haemolytica (2.65%). On the other hand, Gram positive bacterial isolates (n=24) as 6 E. faecalis (5.3 %), 5 E. faecium (4.44%), 4 Streptococci (3.53 %) and 9 S. aureus (7.96%). The results of haemolytic activity of bacterial isolates (selected representatively) from mastitis milk samples on sheep blood agar revealed that 70.5% of the bacterial isolates were haemolytic; 47.6% were ß-haemolytic while 22.9 % were a-haemolytic. Haemolysis was represented as 100%, 90%, 85.71% and 56% in M. haemolytica, Streptococcus spp., Staphylococcus spp. and E. coli, respectively. They were mostly ß-haemolytic 29 isolates (47.6%) while, 18 isolates (29.5%) were ?-haemolysis. The results of haemolytic activity of bacterial isolates from endometritis samples (selected representatively) on sheep blood agar of different bacterial pathogens revealed that 56.8% of the bacterial isolates were haemolytic; 20.3% were a-haemolytic and 36.5% were ß-haemolytic. Haemolysis was represented as 100%, 87.5%, 85%, 61.1% and 40% in M. haemolytica, Streptococcus spp., Staphylococus spp., E. coli and P. aeruginosa, respectively. In addition, 27 isolates were ß -haemolytic with a percentage of 36.5%. Whereas, 32 (43.2%) of the isolates were ?-haemolysis. Biofilm production was represented as 39 E. coli isolates (65%) (18 isolates from mastitis 30% and 21isolates from endometritis 35%), 20 Staphylococci isolates (66.67%) (16 isolates from mastitis 53.33% and 4 isolates from endometritis 13.34%), 7 Streptococci isolates (70%) (5 isolates from mastitis 40%) and 3solates from endometritis (30%), 3P. aeruginosa isolates (42.85%), 3 P. multocida (50%) (1 isolates from mastitis 16.67% and 2 isolates from endometritis 33.33%) and two isolates from endometritis 66.67% and 2 Enterococci isolates (one isolate of E. fecalis from goat and one isolate from E. faecium from sheep) (40%) from uterine samples. The results of in-vitro antimicrobial susceptibility of mastitis milk samples against 17 antimicrobial agents showed high susceptibility against ofloxacin (76%), followed by co-trimethoprim (73.6%), sulfamethoxazole-trimethoprim (71.2%), ciprofloxacin (70.4%), fosfomycin (68.8%), chloramphenicol (67.2 %) and doxycycline (65.6%). On the contrary, most of the isolates were resistant to amoxicillin-clavulanic acid (75.2 %), cefotaxime (72.8 %), amoxicillin (69.6 %), cephalexine (68.8 %), and cefotriaxone (64.8%). All bacterial isolates recovered from uterine samples were tested for in-vitro antimicrobial susceptibility against 17 antimicrobial agents of 9 different antimicrobial classes. The results showed high susceptibility against co-trimethoprim (76.11%) followed by ofloxacin (73.45%), fosfomycin (72.57%), doxycycline (71.68 %), ciprofloxacin (69.91%), sulfamethoxazole-trimethoprim (69.03%), tetracycline (69.02%) and chloramphenicol (67.26%). On the contrary, most of the isolates were resistant to amoxicillin-clavulanic acid (76.11%), cefotaxime (71.68 %), amoxicillin (70.79 %), cephalexine (69.02 %) and cefotriaxone (58.41 %). Using PCR to assess biofilm associated genes and MDR genes in different bacterial isolates as E. coli isolates harbored blaTEM genes (100%), while, qnrA gene was detected in 50% of the tested isolates of ovine endometritis. Meanwhile, all tested isolates harbored fimH virulence genes (100%). The iutA was detected in 25% of the tested isolates of endometritis of sheep only. P. aeruginosa harbored blaTEM, tetA, toxA and las1 with a prevalence rate of 100%. S. aureus isolates harbored mecA and coa (100%). The tetK and icaA genes were detected in 75% of the tested isolates. Streptococci isolates harbored aac(6')aph(2'') gene (100%). The tetO, cylE and cfb were detected in 75% of the tested isolates. P. multocida isolates isolates harbored nanB, toxA and omp87 with a prevalence rate of 100%. M. haemolytica isolates harbored gcp, itkC and ssa with a prevalence rate of 75%. The results concluded that there is a strong correlation between the phenotypes and genotypes of AMR in different bacteria. Moreover, biofilm is a main problem for dairy farms affecting the udder health, uterine tissue and is considered as a risk for milk quality, fertility and consumer health. Also, biofilm formation, in turn, is associated with acquiring MDR by a high prevalence of bacterial