Basic Informations

C.V

        

        Beni-Suef University

Faculty of Veterinary Medicine

           

     Curriculum Vitae

* Personal Information:

Name: Mohamed Ahmed Bakry.

Gender: male.

Date of birth: 3/12/1996

Site of birth: Egypt. Social status: single. Nationality: Egyptian.

Telephone (s): Home          0824408004

Work      +202 / 082 / 2322066

+202 / 082 / 2327982

Cell phone: +2/01005237465 Fax: +202 / 082 / 2327982

Address: AL Doctor Farouq Abd Halim Street, Beba - Beni-Suef Governorate – Arab Republic  of Egypt.

E-mail: mohamedomar31296@yahoo.com

Occupation: Demonstrator of Parasitology – Faculty of Veterinary Medicine, Beni- Suef University, Egypt.

* Educational Background:

? Obtained the degree of Bachelor of Veterinary Medical Sciences

(B.V.Sc) from Faculty of Veterinary Medicine, Beni-Suef University, Egypt, with final grade very good in september 2021.

  

* Professional positions:

? Demonstrator of Parasitology at the Faculty of Veterinary Medicine Beni-

Suef, University, Egypt, in 1/3/2022

* Teaching activities:

? From    2022       teaching the         practical            course    of   Parasitology (Helminthes, Arthropoda and Protozoa) for the under-graduate (third year) and post- graduate students in  Faculty                 of     Veterinary Medicine Beni-Suef,      Beni-Suef University, Egypt.

* scientific events:

1)        Participation in pets online 15 hours course at basics & clinical examination of pet animals medicine.

2)        Participation in pets 15 hours online course in management and most common diseases poultry. 

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* Computer courses and skills:

Training cycle in ICDL

1-    Training cycle in Microsoft Office

2-    Training cycle in Microsoft Office Word

3-    Training cycle in Microsoft Office Publisher.

4-    Training cycle in Microsoft Office Access

5-    Training cycle in Information Technology (IT)

6-    Training cycle in Microsoft Office Outlook

7-    Training cycle in Microsoft Office PowerPoint

                                                                       

                               

Master Title

Screening and control of ectoparasites of pets in Egypt

Master Abstract

6. Summary The current study aimed to detect the surveillance of ectoparasites infesting pets, investigating the occurrence of the ear mites, Otodectes cynotis, in owned cats in 3 provinces, Cairo, Giza and Beni-suef, Egypt, morphometrical recognition of O. cynotis: adults and larval stages, molecular identification of O. cynotis using cPCR as well as phylogenetic analysis of the recovered species, exhibiting the most predominant clinical signs of otitis externa in O. cynotis-infested cats and studying risk factors including age, sex, breed, season and residence, of the recovered mite as well as their effect on the biology of O. cynotis. Specimens were collected from different veterinary clinics using different methods and prepared for morphometry, direct microscopy, molecular identification, phylogenetic analysis and sequencing. Descriptive statistics denoting the effect of risk factors depending on questionnaire owners were done. It has been found that the overall prevalence of ectoparasitism in dogs was 54.90%. One hundred and twelve dogs infested with 3 species of ectoparasites: tick (Rhipicephalus sanguineus), fleas (Ctenocephalides canis, Ctenocephalides felis) and lice (Heterodoxus spiniger). The prevalences of infestation were 80.35, 18.75 and 0.89%, respectively. According to the effect of risk factors on ectoparaistism, dogs aged 1-5 years showed higher infestation rates than those up to one and 5-10 years. No statistically significance was detected P>0.05. Concerning the sex, males showed a higher infestation rate than females in the 3 species of ectoparasites recorded in the study with a detectable statistical significance at P< 0.05. Relative to the breed, long hair-breed (German shepherd) showed a higher infestation rate than short one (Malinois) due to long hair represent haven for ectoparasites. Seasonal variation poses a potential factor in recording the prevalence of ectoparasites. Ticks and fleas had the highest infestation rates in summer, although, fleas infestation becomes higher in winter. Statistical significance was recorded at P< 0.05. Relative to infestations among examined dogs, it has been showed that 112 (54.90%) dogs had at least one ectoparasite species infestation, of which 98 (48.03%) dogs had a single species infestation, which had a significantly higher prevalence than the remaining 6 (2.94%) dogs that had multiple ectoparasite species concurrently infested. The overall prevalence of O. cynotis in examined cats was 25.47%. Risk factors affecting the prevalence of O. cynotis were age, sex and breed of infested cats as well as season. Cats aged 1-6 months were highly susceptible (82; 71.30%), followed by cats aged 6-12 months (40; 54.79%), cats aged 1-4 years (34; 14.71%) and cats aged 4-10 years (18; 6.81%) with a statistical significance at P< 0.05. Out of 402 examined male cats, 23.38 % (94/402) were infested with Otodectes cynotis. Moreover, out of 281 examined female cats, 28.46% (80/281) were infested. No statistical significance was recorded. The highest infestation rate was in autumn (47.61%; 60/126) followed by winter (34.02%; 49/144) and spring (24.12%; 55/228) and the lowest prevalence was found in summer (5.40%; 10/185) with a detectable statistical significance at P< 0.05. According to the breed, it has been found that the highest infestation rate was found in Persian cats (42.15%; 137/325), followed by Baladi cats (28.88%; 13/45), Angora (10.52%; 12/114), mixed breeds (8.16%; 4/49) and Siamese cats (6.25%; 6/96) with the lowest prevalence in Himalayan cats (3.84%; 2/54) with a statistical significance at P< 0.05. Household cats showed infestation rates 38.23% (125/327) and 11.92% (23/193) for both cats bred alone and those bred with other pets, respectively. The infestation rate in outdoor cats was 15.95% (26/163). On recording clinical manifestations and otoscopic examination, thirteen infested cats were symptomless and normal ear discharge despite examination revealed less than 2 mites/field. Mild infestations with 2-5 mites/field exhibited brownish granular ear discharge and pruritis, restless and discomfort. In moderate infestations, cats suffered from head rubbing, erythematous dermatitis and unilateral ear scratching while ear discharge was brownish waxy and 5-10 mites/field were visible. In severe infestations, ear discharge was purulent due to developing secondary bacterial infections and huge numbers of mites/field were detected, referred to that severe ulceration in the ear, head tilting and excessive uni/bilateral ear scratching were clearly detected. Morphology of male, female and developmental stages of Otodectes cynotis was described. The present findings revealed that the adult male was slightly oval with sclerotized dorsal opisthosomal and propodonotal plates. It measured 320 x 269 µm. Gnathosoma was cone-shaped, measured 60µm long with a distinct line of demarcation between it and the ventrocephalic region of the idiosoma. In association with the reproductive system, sclerotized areas are found posterior and lateral to the genital suckers and around the aedeagus.On the metapodosomal region, the male’s ventral surface had a gonopore with two cuticular pits (genital suckers).Pretarsal caruncles (pullvilli) on legs I, II, III, and IV. The adult female measured 450 x 275 µm with being ovoid-shaped. Gnathostoma was 75 µm long. It lacked the genital sucker. The ventral aspect had epigynal apodeme, horse-shoe-shaped epigynium and transverse slit-shaped vulva (genital opening) located between legs II and III. The ovipore located at the middle third of idiosoma. Legs I and II ended with suckers, while legs III and IV terminated with long setae with the leg IV was rudimentary. Egg measured ~197 x~95 µm, elliptical- or subcylindrical-shaped, and more or less flattened on one side. The larva measured ~ 214 x 121 µm, oval-shaped, brown-colored, hexapodous, soft body, slightly larger than the egg and smaller than protonymph with no sexual dimorphism could be detected. Gnathostoma was 40 µm long and similar to that of the adult but smaller. Protonymph showed no sexual dimorphism and smaller than deutonymph. The whole body measured ~ 260 x ~165 µm. Gnathostoma was ~ 35 µm long. Adanal suckers were absent. Legs were easily recognized, with signs of articulation and provided with a long terminal and a smaller subterminal seta. Tritonymph/deutonymph was larger than protonymph but smaller than adult male and female. It measured ~ 297 x 207 µm. Gnathostoma was ~53 µm long. Posteriorly, opisthosoma was weakly bilobed with copulatory tubercles. Legs I, II ended with suckers. Leg III was had two terminal whip-like setae. Leg IV was visible under a higher magnification. By the use of polymerase chain reaction targeting mtDNA COI gene reveled a specific amplicon ~ 655 pb. The phylogenetic analysis showed that the obtained sequence was clustered in the same clade of 14 Otodectes cynotis GenBank isolates. Psoroptes species GenBank isolates were clusterde in a different group. On the contrary, Sarcoptes scabiei GenBank isolates were outside group and rooted the tree. The identity percentage of the obtained sequence of the current study was 100% with Otodectes cynotis GenBank isolates of cat origin (OQ683857, KF891933, KP676682) and dog origin (KP676683 - KP676686). Moreover, it showed 99% identity with O. cynotis cat origin GenBank isolates (MW350132, KP676678- KP676681) and dog origin (KP676687 and KP676688). The nucleotide variations of the obtained sequence compared with the GenBank isolates were illustrated.

PHD Title

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PHD Abstract

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