Basic Informations

C.V

Master Title

pathological studies on external and internal parasitic affections of some ornamental fishese

Master Abstract

Aquarium ornamental fish farming globally is considered as a key sector of aquaculture and represents a significant portion of the pet industry. Between June 2022 and February 2023, a total of 439 ornamental fish specimens including 229 goldfish (Carassius auratus), with mean total length10.75±0.903 cm and body weight ranging from 6.5gm to 129gm and 210 pangasius catfish (Pangasiadon hypophthalamus) with mean total length 11.4±3.37 cm and body weight ranging from 2gm to 29.5gm were examined to investigate the histopathological lesions associated with the parasitic fauna. These ornamental fishes were collected from Elsaieda Aisha market in Cairo governorate, Egypt and some pet shops in Beni-suef and Elfayoum governorates. Gross examinations of the affected fish with different types of the parasites were varied and appeared as red elevated areas, haemorrhages at sites of parasites’ attachment, white spots on the body' with excessive mucus production. Worm-like copepods were protruded on different parts of the body together with ulceration, white colored and pale anemic or congested gills were also common. The overall prevalence of the parasitic infections was 47.2 % in Carassius auratus and the peak of infection with parasites in Carassius auratus was recorded in autumn 54.8% followed by summer 54.5% while the lowest rate was in Winter 20%. In Pangasiadon hypophthalamus, the prevalence was 59.5% and the peak of infection with parasites was recorded in summer 66.7% followed by Winter 66.3% and Autumn 45.7%. The isolated parasites include protozoan parasites (Trichodina sp., Ichthyophthirius multifiliis, Chilodonella sp. and Icthyoboda necator), monogeneans (Dactylogyrus sp. and Gyrodactylus sp.), EMC of Centrocestus fomanus, crustaceans (Lernaea sp. and Argulus sp.) and one nematode Capillaria sp. with incidence rates 27.9%, 15.3%, 2.6%, 12.7% and 0.4% respectively in Carassius auratus and 21.9%, 46.2%, 1.9%, 0% and 0% in Pangasiadon hypophthalamus. Moleular identification of Thaparocleidus caecus from gills of P. hypophthalamus. Histopathological changes included vacuolation, hyperplasia and necrosis of epidermal cells, different sections of parasite between and attached to the gill lamellae, hyperplasia, metaplasia, deformity and fusion of secondary gill lamellae, desquamation and necrosis in both primary and secondary gill lamellae in addition to leucocytic cell infiltrations mainly lymphocytes and macrophages, hyperplasia of gill cartilage and parasitic cyst surrounded by several layers of fibroblast like cells were noticed especially in case of encysted metacercariae. It is worse to mention that, the Encysted metacercariae and nematode (Capillaria) were the only detected internal parasites in the examined fish species. In conclusion, the current results revealed that the prevalence rate of parasites in both species is relatively high as well as the pathological findings induced by these parasites and might negatively affect the proper function of the skin and gills of the host, and could have a negative impact on fish health resulting in significant economic losses in this fish sp. and possibility of transmission to other fishes.

PHD Title

PHD Abstract