Basic Informations
C.V
CURRICULUM VITAE
Hala Sayed Hassan Salam,PhD
Lecturer of Microbiology
Faculty of Veterinary Medicine
Beni-Suef University (BSU)
Beni-suef governorate
Egypt
Date of Birth: 20.01.1977
Place of Birth: Beni-suef
Nationality: Egyptian
Status: Married with three children.
RESEARCH AND PROFESSIONAL EXPERIENCE
Present
Lecturer of Bacteriology, Mycology and Immunology, Faculty of veterinary medicine, Beni-Suef University.
2008-2009
Carrying the practical work of my doctoral thesis in Friedrich Loeffler Institute, Institute of bacterial infection and zoonoses,Jena, Germany in the laboratory of Dr. Helmut Hotzel.
2005 –2007
Assistant lecturer of Bacteriology, Mycology and Immunology, Faculty of veterinary medicine, Beni-Suef University.
2000-2004
Demonstrator (instructor) in Bacteriology, Mycology and Immunology department, Faculty of veterinary medicine, Beni-Suef University.
Degrees
(Sep. 2000)
Bachelor in Veterinary Medicine (B. V. Sc.)
Institution: Faculty of Veterinary Medicine, Cairo University, Beni-Suef branch.
General Grade: Very good with degree of honour
(Oct. 2004)
Master of Microbiology and Immunology, College of Veterinary Medicine, Beni-Suef University.
Institution: Faculty of Veterinary Medicine, Beni-Suef University.
Thesis title: " Bacteriological and immunological studies on Corynebacterium pseudotuberculosis "
(March 2010)
Ph.D. of Microbiology and Immunology,
Institution: Faculty of Veterinary Medicine, Beni-Suef University.
Thesis title" Studies on the bacterial causes of bovine mastitis with special reference to Mycoplasma species''
TECHNICAL TRAINING GRANT
20-Months scholarship in Freidrich-Loeffler Institute, Germany from 11th January 2008- to 29th October 2009 for carrying the practical work of the doctoral thesis.
SOME OF THE TECHNIQUES AND METHODS THAT I AM FAMILIAR WITH
Bacteriological technique
Isolaton and differentiation of difffrent bacteria
Isolation and handling of Mycoplasma.
Molecular diagnostic techniques
Gel electrophoresis
DNA manipulation
PCR and multiplex PCR as well as real time PCR
Southern blot technique
Microarray technique
Different serological techniques
ELISA(Enzyme Linked Immunosorbent Assay)
Haemagglutination inhibition, passive
haemagglutination, agar gel precipitation test (AGPT),
Bioinformatics
Primer design
Nucleotide analysis (Blast, Multisequence alignment)
Responsibilities
1) TEACHING (in English)
1-Teaching Practical and Diagnostic Bacteriology, Mycology and Immunology to undergraduate students (3rd year in faculty of Vet. Med.).
2-Teaching Practical and Diagnostic Bacteriology, Mycology and Immunology to postgraduate students.
3-Teaching and training summer courses of microbiology to undergraduate.
2) RESEARCHING:
My current researches are on:
- Resistance of E. coli from different sources to different antibiotics.
Editions
Editor with other teaching members in the department for
1- Notes on veterinary microbiology.
2- An introduction to practical veterinary microbiology.
Prises
The winner of the best Publication in Beni- Suef University in 2011.
REFEREE NAMES AND ADDRESS:
Dr. rer. nat. Helmut Hotzel
Institute of bacterial infection and zoonoses, Friedrich- Loeffler-Institute, Jena,Germany
Telefon: +49 3641 804-2262
Fax: +49 3641 804-2228
E-Mail: Helmut.Hotzel@fli.bund.de
Adresse:
Naumburger Str. 96 a
07743 Jena
Prof. Dr. /Ismail Abd El- Hafeez Radwan Rahil
Professor and Head of Microbiology Department, Faculty of Veterinary Medicine Beni-Suef University, Egypt.
Phone: +2 082 2322066 / 117
FAX: +2 082 2327982
E-mail: Microbiologist111@yahoo.com
Master Title
bacteriological and immunological studies on Corynebacterium Pseudoturberculosis
Master Abstract
6. SUMMARY
1. Nine isolates of C. pseudotuberculosis were recovered from bacteriological swabs collected from 110 swollen and caseated lymph nodes of clinically affected sheep. For the isolation purpose, BHIA and blood agar media were utilized.
2. All the isolates were identified by microscopical, colonial morphological and biochemical characters.
3. All identified isolates were confirmed for the production of the exotoxin PLD using modified CAMP test.
4. Serum samples were collected from 32 diseased sheep showing clinical lesions suspected to be caseous lymphadenitis, 26 apparently healthy sheep but living in contact with clinically affected sheep and from 20 apparently healthy sheep living in free area (less than 6 months old and act as control negative group). These serum samples were subjected to ELISA assay by using 2 types of coating antigens (PLD and somatic antigen). The obtained sensitivities and specificities of the two applied antigens were 100% and 91.7% for PLD antigen, 62.5 and 50% for somatic antigen respectively as regards to the examined 32 clinically affected sheep serum samples.
5. The effect of different antigens (vaccines) on macrophage activity was studied by designation of 4 groups of Balb/C mice each group composed of 5 mice. Four antigens (toxoid, bacterin, living C. pseudotuberculosis and combined vaccine) were used for immunization of these groups of mice.
6. Peritoneal macrophage were collected from the immunized mice groups then transferred to normal mice (recipient mice) which were challenged by living C. pseudotuberculosis (2x106), then macrophages were collected to determine the killing assay of macrophages collected from each group.
7. The killing assay was performed by collection of peritoneal macrophages challenged with the live bacteria and equal number of macrophages collected from each group were lysed and cultivated to count the number of liberated living corynebacterium.
8. The results of the experiment revealed that, the highest killing assay was detected in mice group vaccinated with toxoid then combined vaccine (bacterin plus toxoid) then mice group immunized by living C. pseudotuberculosis and the lowest killing assay was detected in mice group vaccinated by bacterin.
PHD Title
Studies on the bacterial causes of bovine mastitis with special reference to Mycoplasma species
PHD Abstract
7. SUMMARY
Mastitis is the most prevalent and expensive disease on a dairy farm
Knowledge of the prevalence and distribution of mastitis pathogens is critical to the prevention of the disease
Different bacteria were isolated from 111 quarters out of 126 infected quarters
In the present study different bacteria were isolated from 111 QMS out of 126collected from cases of clinical mastitis in Beni-Suef Governorate with an incidence of 88.1%. E. coli was the predominant isolate followed by S. aureus.
The incidence of udder infection with Mycoplasma in a large dairy herd in Germany with a previous history of Mycoplasma mastitis was detected by mycoplasma culture method and digitonin sensitivity. From the total examined animals (1025), 100 Mycoplasma infected animals was detected revealing 9.8% of prevalence on the animal base. On the other hand, the number of Mycoplasma infected quarter were 165 from the total examined quarters (n=4046) as 4.0% prevalence
Confirmation and specification of the isolates was carried by species specific PCR .The three bovine pathogens M. bovis, M. californicum and M. bovigenitalium were detected. The majority of isolates were identified as M. bovis (90.0%). Additionally, 25 of culture-positive and digitonin negative isolates could not be differentiated by subsequent species-specific PCR.
The specificity of the used species specific PCR has been proofed by
Alignment of each primer pair using a multiple sequence alignment computer program with the sequences of reference strains of Mycoplasma species most commonly isolated from milk retrieved from the GenBank. In addition to sequencing of PCR product of one field strain from each Mycoplasma species detected in the current study.
A new Real time PCR for diagnosis of M. bovis directly in milk has been developed by testing 3 different panels of samples, one of them was involved from the current study (n=36).
Investigation of these quarter milk samples (n=36) using Mycoplasma culture confirmed by species specific PCR as traditional method and quantitative RT PCR using DNA extracted by 3 different methods revealed the following. M. bovis positive samples were 18 (50%), 28 (77.8%), 18 (50%) and 9 (25%) for Mycoplasma culture, RT PCR using DNA extracted by procedure 1, 2 and 3 respectively. Such comparison showed that the isolation of M. bovis from milk containing between 100 and 1000 cfu /ml was rarely successful (1 out of 8), whereas the vast majority of higher-titre samples (17/20) were successfully cultured. The negative culture results in this group of samples were due to overgrowth by other bacteria.
The same RT PCR was used successfully to detect M. bovis in group animal milk samples (16-17 animal) and tank milk samples.
Arbitraly primed PCR or Random amplified polymorphic DNA (RAPD fingerprinting has been used to detect the diversity of some M. bovis isolates and all M. californicum isolates.
The conditions of this system were optimized before starting the actual work by adjusting the amount of MgCl2 for each primer (5 primers).
DNA fingerprint patterns obtained by RAPD of M. bovis and M. californicum DNA were analyzed using BioNumerics software (Applied Maths, Sint-Martens-Latem, Belgium).
The number of different band patterns were obtained with each primer taken as a measure of the discriminating power of the RAPD assay.
The highest diversity for M. bovis and M. californicum was detected by primer 1247 and HUM 1 respectively.