Nitric oxide and lysozyme activities as early monitors of the immune response of Brucella abortus RB51 vaccinated cows.

Research Abstract

Nitric oxide and lysozyme activities were estimated in sixty one Brucella abortus RB51 vaccinated cows during the first week post vaccination as well as in four non vaccinated cows. It was quite clear that there was a significant difference (P< 0.001) between the level of nitric oxide in vaccinated (14.61 ± 0.63) and non-vaccinated (2.60 ± ).0.0cows. There was also a significant difference (P< 0.001) between the level of lysozyme in vaccinated (16.26 ± 0.30) and non- vaccinated (8.280+- 0.007) cows. The results obtained in this study revealed that both nitric oxide and lysozyme activities can be effectively used for early monitoring of the immune response of Brucella abortus RB51 vaccinated cows. The results also confirmed that complement fixation test using a rough antigen prepared from the vaccine strain with depriving the anti-complementary activity through incubating the antigen overnight with bovine Brucella antigen serum is suitable and effective to identify RB51 vaccinated cows.

Research Keywords

Brucella, vaccine