Investigation of phenotypic variations amongst Mycoplasma bovis field German isolates using SDS-PAGE

Research Abstract

Mycoplasma bovisis a major bacterial pathogen causing widespread respiratory disease, mastitis, and arthritis in cattle.Ineffective drugs treatments, culling of infected cows andloss of milk production can lead to significant economic losses in dairy farms. The earlydetection of cows excreting M. bovisbacteria to prevent mastitis outbreak is necessary. Inour study the percentage of M. bovisinfection among dairy herd was recorded by cultivationin combination with digitonin test and confirmed by species specific PCR as 4%. Theprotein profile of the isolated field German strains was compared to M. bovisreferencestrain Donetta PG45 using SDS-PAGE. The pattern of the reference strain was different fromthat of field strains as well as a marked heterogenicity among the field strains was noticed.

Research Keywords

Mycoplasma bovis; SDS-PAGE; Species specific PCR