In vitro and in vivo effect of Citrus limon essential oil against sarcoptic mange in rabbits
ملخص البحث
Abstract The effect of lemon oil (Citrus limon)onSarcoptes
scabiei var. cuniculi was evaluated in vitro and in vivo. The
mite samples were collected from naturally infected rabbits.
The lemon oil was prepared in six concentrations by dilution
with distilled water (2.5, 5, 10, 20, 50, and 100 %). In vitro
application was done in five replicates for each concentration
in petri dishes in the laboratory. The treated mites were observed
at 1, 12, and 24 h post application (PA) for lemon oil
effect. In addition, oxidative stress profile was evaluated for
the treated mite. Dependent on in vitro results, 20 % lemon oil
was used in vivo trial. Twenty-four naturally infected rabbits
were divided into three groups of eight: 20 % lemon oil, deltamethrin,
and untreated control. The infected parts of rabbits
were treated topically once a week for four successive weeks.
In vitro application results showed that lemon oil 10 and 20 %
diluted in water caused mortality to 100 % of mites after 24 h
PA. The oxidative stress profile revealed that mites treated
with 20 % lemon oil had significantly (P< 0.05) higher
hydrogen peroxide and malondialdehyde when compared
with mites treated with deltamethrin or distilled water.
In vivo application of 20 % lemon oil on naturally infected
rabbits showed complete recovery from clinical signs, absence
of mite in microscopic examination from the second
week of treatment. In addition, productive performance was
significantly better than infected untreated group. Also, the
treated tissue showed stoppage of scale formation and hair
growth faster than deltamethrin-treated rabbits. Consequently,
lemon oil has remarkable miticidal activity in vitro and in vivo
applications.
الكلمات المفتاحيه
Keywords Rabbits . Sarcoptes scabiei var. cuniculi . Citrus lemon . In vitro . In vivo . Oxidative profile