Effect of Bactocell® and Revitilyte-Plus tm as Probiotic Food Supplements on the Growth Performance, Hematological, Biochemical Parameters and Humoral Immune Response of Broiler Chicken
Research Abstract
This
study was designed to compare between the effect of Bactocell® and
Revitilyte-Plus TM as probiotic supplements on the growth performance,
hematological and biochemical constituents of blood and humoral immune
response of broiler chickens. Three hundred, one day old broiler chicks
were divided into three equal groups, first group (C) fed on a balanced
ration and considered the control group, second group (L) fed on a
balanced ration and provided with the probiotic Revitilyte-Plus TM at a
rate of 1 gm/4 liter of drinking water and the third group (P) fed on a
balanced ration supplemented with the probiotic Bactocell® at a rate of
1kg/ton ration. On the 5th week of age, each of the previous groups was
subdivided into 2 equal subgroups. One of which (C+S, L+S and P+S) is
infected with salmonilla kenticky (8x1012 microorganisms/ml for 2
successive days given in (1ml in the first day and 0.5 ml in the second
day). he experimental groups became (group C, L, P and C+S, L+S and
P+S). Groups C+S, L+S and P+S were infected with Salmonella kentucky (8 x
1012 /ml) for 2 successive days (1ml in the 1st and 0.5 ml in the 2nd
day). The period of the experiment extended for 2 weeks after infection
through which at intervals throughout the time of the experiment, body
weight, food conversion rate, hemogram, some serum biochemical
parameters and humoral immunity using ELISA technique were investigated.
Results revealed significant increase in body weight and FCR in the non
infected groups L and P on the 3rd week and in group P till the end of
the experiment,while the infected groups showed significant decrease in
body weight, along the experiment. Hemogram of the infected groups
revealed macrocytic hypochromic anemia, leucocytosis associated with
heterophilia, lymphocytosis and monocytosis. Serum biochemical
parameters showed elevated Aspartate amino transferas and Alkaline
phosphatase, hypoglycemia, elevated total proteins and globulins,
hypocholesterolemia (HDL and LDL) and low triglycerides when compared
with the control. Results of ELISA assay revealed significant elevation
in humoral immune response in the P+S, L+S and C+S group respectively,
the study concluded that use of probiotics improve the immune response
of birds against salmonilla infection and Bactocell® is better than
Revitilyte-PlusTM as it increased BW, improved FCR, caused low
mortalities and induced more immunoglobulins
Research Keywords
compare between the effect of Bactocell® and Revitilyte-Plus TM as probiotic supplements on the growth performance, hematological and biochemical constituents of blood and humoral immune response of broiler chickens