البيانات الاساسيه

السيره الذاتيه

سيرة ذاتية
الاسم احمد عمر عبد اللطيف
الوظيفة مدرس مساعد الفيروسات- كلية طب بيطرى – بنى سويف
العنوان المنيا
المؤهلات العلمية
دكتوراة – جامعة بوردو بامريكا
ماجستير الفيروسات – جامعة بنى سويف

عنوان رسالة الماجستير

دراسات عن مرض البوردر فى الأغنام

ملخص رسالة الماجستير

Seroprevalence of pestivirus infections in 40 sheep flocks within El-Minia and Beni-Suef Governorates was conducted using ELISA. All examined flocks were found to be positive for pestiviruses. Nine foeti and neonates from sheep and goats in Egypt were screened for pestiviruses using immunohistochemistry (IHC), virus isolation, and reverse transcription-polymerase chain reaction (RT-PCR). Two goat kids with typical border disease manifestations showed positive results with IHC using polyclonal anti-BDV serum but not with four different monoclonal antibodies (Mabs). On inoculation in MDBK, a cytopathic bovine viral diarrhea virus (BVDV) was isolated from one kid. Further sequencing of of 5′-UTR, Npro, and E2 genes revealed that the isolate was identified it as BVDV subtype 1b. Although the circulating virus in Egypt is considered to be BVDV 1a, this report confirms the existence of BVDV 1b in Egypt. Mutations were found in E2 genomic regions which might be the responsible for escaping of the virus for detection with Mabs that targets epitopes on E2 protein. To our knowledge, this is the first report of isolation of a pestivirus from goat in Egypt and is probably the second report worldwide of a goat kid showing central nervous signs associated with border disease.

عنوان رسالة الدكتوراه

ب

ملخص رسالة الدكتوراه

ب