Basic Informations

C.V

NOHAIR AHMED MAHMOUD

Department of pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511, Egypt

Mobile Phone: +20 1092853963

E-mail: nohairahmed98@gmail.com

POSITION

Jan 2023– present

  Demonstrator at Pathology Department, Faculty of Veterinary Medicine ,Beni-Suef

             University, Egypt.            

EDUCATION

Bachelor of Veterinary Medical Sciences (B.V.Sc),May 2021 with a general grade: very good (with Distinction Honor),Faculty of Veterinary Medicine ,Beni-Suef University .

RESEARCH EXPERINCE

 Proficient in tissue processing, including the preparation of formalin-fixed paraffin-embedded (FFPE) specimens for histopathological analysis.

 Proficient in performing and analyzing tissue samples using routine histopathological stains (H&E) and special stains as well.

 Skilled in performing Immunohistochemistry techniques (IHC).

  Skilled in molecular biology techniques such as Polymerase Chain Reaction (PCR).

 Hands-on expertise in microscopic examination of tissue sections using a light microscopy.

 Skilled in interpreting histopathological findings under a microscope and documenting these findings with accuracy and attention to detail.

 Well-versed in electron microscopy techniques.

  Experienced in conducting post-mortem examinations and collect diagnostic samples.

  Skilled in performing frozen section procedures using a cryostat for rapid microscopic analysis.

TECHNICAL AND ANALYTICAL SKILLS

 Proficient in data analysis and visualization using tools such as Microsoft Office (Excel, Word, PowerPoint) and SPSS for statistical analysis.

 Skilled in preparing high-quality imaging documents and visual presentations using Photoshop.

 Experienced in image analysis using ImageJ software for precise and detailed data interpretation.

WORKSHOPS AND TRAINING COURSES

  Completion training in: Statistical Basis and Analysis with SPSS, faculty of veterinary Medicine , Beni-

Suef university, Egypt. | Nov 2024

 Egyptian Knowledge Bank & International Databases Workshop Digital Library, Supreme Council of Universities | October 2024

 International Publication Workshop Faculty of Veterinary Medicine, Beni-Suef University

 Fundamentals of Digital Transformation (FDTC) Certificate Beni-Suef University, Accredited by the Supreme Council of Universities | July 2024

    Training Course in the Uses of Electron Microscope in the field of Research Diagnosis and Applications of Nanotechnology Assiut University – Electron Microscope Unit | February 2024

  Scientific Research and PCR Applications Faculty of Veterinary Medicine, Beni-Suef University

    Plagiarism Course Beni-Suef University | February 15-16, 2023

    Qualification to Work in University Course Human Resources Development Center, Beni-Suef University

| February 20-22, 2023

PRE-MASTERS COURSES

General Pathology: Very good              Biochemistry: Very good

General Toxicology: Very good            Laboratory diagnosis and toxicology: Excellent

VOLUNTEER EXPERIENCE

    Coordinator – Egyptian Health Council Faculty of Veterinary Medicine, Beni Suef University

-Coordinating activities for graduating students to obtain their professional practice licenses.

    HR Head – Food Quality Students’ Association (FQSA) Beni- Suef University, Egypt

-Organizing trainings and conferences related to food quality and safety for university students.

 Event Organizer – Educational Field Trip to the National Animal Production Complex - Youssef El-Seddiq, Fayoum

CONFERENCES

Participating in attendance the” Environmental pollution :

Challenges and Managements “conference, (2024-2025)

LANGUAGES

Arabic : Native English : Fluent REFERENCES

DR Mahmoud EL-Begawy : Professor of

Veterinary Pathology at Faculty of Veterinary Medicine, Beni-Suef University .

E-mail : Mahmoud.begawy@vet.bsu.edu.eg DR EL-Shaymaa Nabil :Professor of Veterinary Pathology at Faculty of Veterinary Medicine, Beni-Suef University .

Master Title

Pathological studies on the use of mesenchymal stem cells associated with exosomes against nanoparticles-based toxicity in laboratory animals

Master Abstract

Summary and conclusion Carbon nanotubes (CNTs), particularly multi-walled CNTs (MWCNTs), are widely used in biomedical applications due to their unique physicochemical properties. However, their ability to cross cellular barriers and interact with intracellular components also contributes to significant toxicity, mainly through oxidative stress, inflammation, and apoptosis, leading to organ damage including neurotoxicity. Mesenchymal stem cells (MSCs) and their derived exosomes (MSC-Exos) have emerged as promising therapeutic strategies owing to their immunomodulatory, anti-inflammatory, and regenerative properties, offering a safer and more effective alternative to counteract nanomaterial-induced toxicity. Thirty-two male albino rats (4 weeks old, ~110 g) were divided into four equal groups (n = 8 each): 1. G1 (Control): received saline intraperitoneally (IP). 2. G2 (MWCNT): received IP injections of MWCNTs (1 mg/kg BW) during the 1st and 3rd weeks. 3. G3 (MWCNT + MSCs + Exosomes): received MWCNTs (as in group 2) plus weekly MSCs (2.5 × 106 cells/kg BW, IP) and exosomes (100 µg protein/kg BW, IP) during weeks 2–4. 4. G4 (MSCs + Exosomes): received MSCs and exosomes only, without MWCNTs. After 30 days, rats were euthanized and lung and brain tissues were collected for: The experimental assessments included a wide range of histopathological and molecular investigations to evaluate the toxic effects of MWCNTs and the potential therapeutic impact of MSCs and their derived exosomes. Histopathological examination was performed using hematoxylin and eosin (H&E) staining for general tissue architecture, Masson’s trichrome for collagen deposition and fibrosis, and periodic acid–Schiff (PAS) for detecting mucopolysaccharides and goblet cell changes. Biochemical assays were conducted to measure oxidative stress and antioxidant status, including malondialdehyde (MDA) as a marker of lipid peroxidation, superoxide dismutase (SOD) activity, reduced glutathione (GSH), and total thiols. Gene expression analysis was applied to investigate inflammatory and fibrotic pathways: TGF-ß1, MMP-9, and NF-?B in lung tissue, while in the brain tissue MAPK, c-Myc, and integrin signaling pathways were examined to explore mechanisms of neurotoxicity and neuroinflammation. In addition, digital morphometry using ImageJ software was employed to provide quantitative measurements of fibrosis area, bronchiolar epithelial thickness, and goblet cell hyperplasia, thus supporting the histopathological findings with precise morphometric data. Lungs: Exposure to MWCNTs induced marked pathological alterations in lung tissue, including bronchiolar epithelial thickening, goblet cell hyperplasia, and extensive fibrosis. These histopathological changes were associated with a significant increase in oxidative stress, evidenced by elevated levels of malondialdehyde (MDA), alongside the upregulation of pro-inflammatory and profibrotic genes such as TGF-ß1, MMP-9, and NF-?B. In contrast, treatment with mesenchymal stem cells (MSCs) and their derived exosomes markedly alleviated these alterations. Fibrosis and epithelial remodeling were significantly reduced, oxidative stress was mitigated, antioxidant defenses (GSH, SOD) were restored, and pro-inflammatory gene expression was downregulated, indicating strong protective and regenerative effects. Brain (hippocampus and cortex): In the central nervous system, MWCNT exposure led to evident neurotoxic effects characterized by nuclear pyknosis, neuronal atrophy, and a reduction in neuronal density in the hippocampal and cortical regions. These structural changes coincided with redox imbalance, reflected by increased MDA and decreased SOD activity. Molecular analysis further revealed upregulation of MAPK and c-Myc pathways along with impaired integrin signaling, suggesting activation of stress and apoptotic pathways contributing to neurodegeneration. Administration of MSCs and exosomes substantially alleviated neuronal degeneration, improved antioxidant status by restoring redox balance, and downregulated MAPK and c-Myc expression while enhancing integrin signaling. These findings collectively demonstrate the neuroprotective potential of MSC-based therapies against MWCNT-induced neurotoxicity. Conclusion: Based on this study, MWCNTs exerted pronounced pulmonary and neurotoxic effects mediated by oxidative stress, inflammation, fibrosis, and gene dysregulation. Co-administration of MSCs and MSC-derived exosomes provided significant protective and regenerative effects by restoring antioxidant capacity, reducing fibrosis, and modulating inflammatory and survival pathways. these results further highlight the potential adverse health effects that may occur following MWCNT exposure and therefore we suggest these materials may pose a significant risk leading to impaired lung function following environmental and occupational exposures. And also highlight the therapeutic potential of MSCs and MSC-Exos against nanomaterial-induced toxicity and the value of digital pathology in nanotoxicology assessment.

PHD Title

no thesis

PHD Abstract

no thesis