Basic Informations

C.V

Personal details

Name: Abeer Mohammed Radi

Nationality: Egyptian

Date of birth: 28-1-1975

Material status: Married

Address: Pharmacology Dept. Fac. Vet. Med. Beni-Suef University

Telephone:

Mobile 01155128282

Work 0822327982 (Tel/Fax)

E-Mail:, rouqaa2007@yahoo.com abeer.radi@vet.bsu.edu.eg

Educational background

Date Qualification and Institution

1997 Bachelor degree in Veterinary Medicine

(Faculty of Veterinary Medicine, Cairo University, Beni-Suef )

2002 Master degree in Vet. Pharmacology

(Faculty of Veterinary Medicine, Cairo University, Beni-Suef )

2008 Ph.D. thesis Vet. Pharmacology

(Faculty of Veterinary Medicine, Cairo University, Beni-Suef )

Career History

Date Job and Institution

1998-2002 Demonstrator of Pharmacology

(Faculty of Veterinary Medicine, Cairo University, Beni-Suef )

2002-2008 Assistant lecturer of Pharmacology

(Faculty of Veterinary Medicine, Cairo University, Beni-Suef )

2008 to date Lecturer of Pharmacology

(Faculty of Veterinary Medicine, Cairo University, Beni-Suef )

RESEARCH

Current research is on pharmacodynamics and pharmacokinetics of drugs and plants.

TEACHING / INSTRUCTION

Pharmacology (Pharmacodynamics and Pharmacokinetics of drugs)

Publications:

1-Zaghloul, H.A.H., El-Gendy, A.A.M., Tohamy, M.A. and Radi, A.M. (2007): Effect of sulphadoxine-trimethoprim combination on some pharmacokinetic aspects of sulphadoxine in goats. BS. VET. MED. J., 17(1): 78-85.

2-Tohamy, M.A. and Radi, A.M. (2008): Bioavailability and pharmacokinetics of florfenicol in healthy foals. J. Egypt. Soc. Pharmacol. Exp. Ther., 29(2): 539-551.

3-A.A.M. El-Gendy, M.A. Tohamy and Abeer M. Radi (2009): Pharmacokinetic profile and some pharmacodynamic aspects of cefquinome in chickens. BS. VET. MED. J., 19(2): 33-37.

4-Radwan.A.I and Abeer, M.Radi ( 2010):Antimicrobial activity of some cephalosporins with special reference to their effects on body weight and immune response to new castle disease vaccine in fayoumy chiks . beni-suief veterinary medical journal 6th sci-conf. vol.20,No1,p.236-242.

5-A.A.M. El-Gendy, M.A. Tohamy and Abeer M. Radi (2011): Some pharmacological studies of cephradine in broilers. BS. VET. MED. J.,.

6-Ghada, M. Safwat and Abeer, M. Radi (2011): Evaluation of the antioxidant protective effect of oxytocin and silymarin against gentamycin- induced nephrotoxcicity in rat. BS. VET. MED. J. vol(21),No.1:1-7.

7- Abeer.M.Radi (2012): pharmacokinetics and bioavailability of spectinomycin in sheep., Suez canal Veterinary medicine journal(SCVMJ, XVII(2):161-169.

8- F.I. Abo El-Elaa, A.M. Radia*, H.A. El-Bannab, A.A.M. El-Gendya & M.A. Tohamy (2014):Pharmacokinetics of di?oxacin in healthy and E. coli-infected broiler chickens. British poultry science journal, Volume 55, Issue 6, pages 830-836.

9- Radwan, I.A., Abed, A.H., Abeer, M.Radi, Ibrahim, M.,A., Abdallah, A.S.(2014): Effect of Thyme, Clove and Cinnamon Essential Oils on Candida Albicans and Moulds Isolated From Different Sources. American journal of animal and veterinary science, 9 (4): 303-314

10- Abeer M. Radi ; Naglaa M. Abdel Azeem; EL-Shaymaa EL-Nahass; Fatma Khalil (2014): Effect of a macrolide antibiotic tulathromycin on fertility of male albino rats. Veterinary Medical Journal- Giza, Vol. 60, (2), 2014.

11- Abeer M. Radi and Nesreen A. Ibraheem (2015):

Comparison of efficacies of Fosfomycin, Thiamphenicol , Nigella sativa oil and its against Escherichia coli infection in chickens.

Assiut Veterinary Medical Journal, Vol. 61, No. (144), January 2015.

: Professional Experience

*Teaching Experience

1999\2010: Teaching Practical Pharmacology to 3^rd year undergraduate students and designing laboratory experiments and exams for students.

– Faculty of Veterinary Medicine -Beni-suef university

2009 - present: lecturer, Department of Pharmacology, Faculty of veterinary medicine ,Beni -Suef University. Teaching Pharmacology to 3^rd year undergraduate students . Teaching Practical Pharmacology to postgraduate students and designing laboratory experiments and exams for these students.

1998-present :training of the students on clinical pharmacology in summer training programme, in, Department of Pharmacology, Faculty of veterinary medicine, Beni Suef University, Beni Suef.

*Training programs

1-(Faculty & Leadership Development Project; FLDP, Beni Suef University, Beni suef, Egypt):

Effective presentation
Strategic Planning
International publishing of research

4-Use of technology in teaching

5- University Administration

2- Softwares: Excellent computer knowledge, MS office passed ICDL with excellent scores.

Manusrcipts in preparation

Conferences and Meetings

1- Fifth international conference(Towards Safe Food in Egypt),by The Egyptian Society of environmental Toxicology, (2009).

2- international scientific conference, faculty of Veterinary medicine, Beni-Suef University, (2010 ).

3- Training course organized by toxicological& Forensic Medicine Department , faculty of veterinary medicine, Cairo university, Egypt. In cooperation with united states Environmental protection Agency(VS-EPA).Risk assessment as a critical tool for everyday's challenges.(2012).

4- 7^th international scientific conference, faculty of Veterinary medicine, Suez canal University, (2012).

5- preparing competitive project for research funding,(2013).

6- Training course in “nanoscience and nanotechnology”,

Faculty of postgraduates for advancen sciences. Beni-Suef

University, (2013).

7- The 4^th international scientific conference, faculty of Veterinary medicine, Cairo University, (2014).

Master Title

Effect ofdrug interaction on ome aspects of sulphadoxine in goats

Master Abstract

SUMMARY ******** This work was carried out to reveal the pharmacokinetic parameters, clearance and creatinine clearance of long acting sulphonamide, sulfadoxine when given alone and in combination with trimethoprim. The effect of both drugs on concentrations of creatinine was also studied. This study was carried out on 12 balady female goats weighing of 20-25 kg and of 18 to 24 months old and proved to be clinically healthy. The goats were divided into four equal groups.The first group was injected sulfadoxine alone intravenously in a dose of 15 mg/kg b. wt. The second group injected sulfadoxine intramuscularly in the same dose of intravenous injection. The third group was injected sulfadoxine and trimethoprim combination intravenously in a dose of (15 mg/kg b. wt.+3 mg/kg b.wt.). The fourth group injected sulfadoxine and trimethoprim combination intramuscularly in the same dose of intravenous injection. Blood, urine samples were collected from goats just before and 5, 10, 15, 30 minutes, 1, 2, 4, 6, 8, 12, 24, 48, 72, 96, 120 hours post –injection to study their pharmacokinetic profiles. 1-Single intravenous injection: After intravenous injection of sulfadoxine in a dose of 15 mg/kg b. wt. , the drug was showed a high serum level ( 96.46 ug/ml )5 minutes post-injection, then the concentration was decreased gradually till reached its minimum level ( 1.66 ug/ml) at 120 hours post-injection. While after intravenous injection of sulfadoxine and trimethoprim in a dose of 15 mg/kg b.wt.+3mg/kg b.wt. , the drug was showed a high serum level (93.95 ug/ml) 5 minutes post-injection. Then the concentration was decreased gradually till reached its minimum level (1.24 ug/ml) at 120 hours. The present data revealed that the serum concentration time curves of sulfadoxine were best described by two compartment –open model following a single intravenous injection of 15 mg/kg b. wt. The drugs were distributed with a distribution half –life (t1/2 a) of 2.48hour and 2.31 hour, respectively, and the apparent volume of distribution calculated by area [Vd (area)], extrapolation [Vd(B)] and steady state [Vd(ss)] methods were 384.71±2.19,317.59±3.48 and 368.54±2.99 , respectively for sulfadoxine and 414. 67± 3. 29, 414. 53±000, 420. 29±5. 16 (p<0.01) and 411. 73±3. 48 ml/kg, respectively for sulfadoxine –trimethoprim combination . The drug was distributed in the central compartment with a volume of distribution (Vc)(153.31±2.67 , 156. 85±3.17 ml/kg), respectively. This indicated that trimethoprim increase distribution of sulfadoxine in the body . The drug was eliminated with a half-life of elimination (t1/2 ß) of (23.10 hour and 24.75 hour) for sulfadoxine alone and sulfadoxine –trimethoprim combination, respectively .Body clearance (Cl B)were 0.076±0.004 and 0.073 ±0. 008 l/kg/h, respectively. This indicating that trimethoprim enhance the excretion of sulfadoxine . The concentrations of the drugs in the urine were much higher than its concentration in serum. It were 876.618 and 1328.029ug/ml, respectively at 30 min. post-injection and was reached its maximum level ( 1178.25 and 1505.8 ug/ml )at one hours post-injection and then decreased gradually to reach its lowest concentration(33.5 and 15.278 ug/ml) ,respectively at 120 hours post-injection(p<0.05). The percentage of N4-acetylated derivatives of sulfadoxine was higher in urine than in serum and with sulfadoxine trimethoprim combination than in case of sulfadoxine alone , It reached its maximum levels (60.667%in serum after 72 hours and , 65.216% in urine after 96 hours in goats injected with sulfadoxine and trimethoprim. Whereas it was 50.672%in serum at (48 hr) and 58.33%in urine at (120 hr)in goats injected sulfadoxine alone .This result indicated that the trimethoprim enhance the metabolism of sulfadoxine through acetylation . The rate of sulfadoxine clearance from goat’s blood after a single intravenous injection of both drugs was decreased by time post injection . The creatinine concentrations in serum and urine of goats following a single intravenous injection of 15 mg/kg b.wt. revealed no marked significant altrations. The ratio between sulfadoxine clearance from goats blood to endogenous creatinine clearance was ranged from 0.089-0.260 and 0.173-0.469 following intravenous injection, this value indicated that the glomerular filtration is the main route of excretion of sulfadoxine through goat’s kidney. 2-Single intramuscular injection : Following intramuscular injection of sulfadoxine in a single dose of 15 mg/kg b. wt. , the drug was detected in serum 5 min. post –injection (2. 11ug/ml) .It continued to increase gradually till reached its maximum level ( 26. 18 ug/ml) at 2 hours post-injection. Then it was started to decreased gradually till reached its lowest concentration(0.88 ug/ml) at 120 hours post-injection. In case of and intramuscular injection of sulfadoxine –trimethoprim combination the drug was detected in serum (4.39ug/ml) 5 min. post –injection(p<0.05) and it continued to increase gradually till reached its maximum level (33.52 ug/ml) at 2 hours post-injection (p<0.05), then started to decreased gradually till reached its lowest concentration (0.65 ug/ml )at 96 hours post-injection(p<0.01). The drug was absorbed with a half-life of absorption (t1/2 ab ) of 0.58 hour and 0.42 hours (p<0.05), respectively. This indicating that trimethoprim increase the rate of absorption of sulfadoxine. While its elimination half-life (t1/2 el ) was 23. 89 hours and 17.325 hours (p<0.05) ,respectively. This revealed that trimethoprim enhance the excretion and metabolism of sulfadoxine . The concentration of the drug in urine was much higher than its concentration in serum. It were 235.65 and 325.197ug/ml,respectively at 30 min. , then increased to reach its highest level (1371.483 ug/ml)at one hour post-injection for sulfadoxine alone and reached 763.506ug/ml after 2 hour for sulfadoxine –trimethoprim combination. Then decreased gradually to reach its lowest level (45.132 ug/ml at 120 hours and 15.973 ug/ml at 96 hours post injection(p<0.01) , respectively ) . The percentage of N4-acetylated derivatives of sulfadoxine was higher in urine than in serum and with sulfadoxine trimethoprim combination than in case of sulfadoxine alone , It reached its maximum levels (64.7%in serum(p<0.05) and 72.75% in urine)after 72 hours in goats injected with sulfadoxine and trimethoprim. It was (53.024%in serum ,60.15%in urine )at 72 hr in goats injected sulfadoxine alone .This result indicated that the trimethoprim enhance the metabolism of sulfadoxine through acetylation . The rate of sulfadoxine clearance from goats blood after single intramuscular injection of sulfadoxine and sulfadoxine - trimethoprim combination were decreased by time post- injection. The creatinine concentrations in serum and urine of goats showed no significant alteration ,except at 6 hour there was significant decrease (p<0.05) in case of combination . The creatinine clearance expressing the glomerular filtration rate from goats blood was showed significant increase following intramuscular injection of sulfadoxine - trimethoprim combination (p<0.05). The ratio between sulfadoxine clearance to endogenous creatinine clearance was ranged from 0.285-0.492 and 0.064-0.253, respectively following intramuscular injection of sulfadoxine alone and sulfadoxine - trimethoprim combination. This value indicated that the glomerular filtration is the main route of execration of sulfadoxine through goat’s kidneys.

Master Abstract PDF

PHD Title

Some pharmacological studies of some antibiotics in broilers

PHD Abstract

Summary ********* The present work was carried out to reveales some pharmacokinetic aspects of two antibacterials, cephradine and cefquinome in chicken .The bioavailability of cephradine following intramuscular ,subcutanious injection and oral adminstration were estimated . The protien binding percent of both drugs to serum were also estimated. Also tissue distribution and residues of cephradine after repeated oral administration and cefquinome after repeated intramuscular injection for 5 consecutive days were also studied. The in-vitro susceptibility of isolated microorganism from one old day chicks towards the two antibacterials were also investigated. The effect of both antibacterials on serum liver enzymes activity and concentration of uric acid in serum following repeated administration were also studied. The present study was carried out on 114 balady chicken and 50 one day old Fayomy chicks. These birds were obtained from El.Azab project for poultry production in Fayoum Governorate with an avearage body weight from 1.280 to 2.800 kg and aged from 4-12 monthes and proved to be clinically healthy. In the first experiment 72 chickens were classified into six groups each of 12 chickens. The 1st , 2nd ,3rd and 4th groups were injected cephradine in a single dose of 50 mg kg-1b.wt. via oral, intramuscular, subcutaniously and intravenously respectively . Chickens of the 5th and 6th group were injected cefquinome in a single dose of 2 mg kg-1b.wt. intramuscularly and subcutaniously respectively. Blood samples were collected 5, 10, 15 and 30 min. 1, 2, 4, 6, 8, 12 and 24 hours post injection. The concentration of both drugs in serum sample was determined using the microbiological assay method. In the second experiment tissue distribution and residues of cephradine in a dose of 50 mg kg-1 b.wt after repeated oral administration in 24chicken twice daily every 12 hours and cefquinome after repeated intramuscular injectionof 2 mg kg-1b.wt. in 18 chicken once daily for 5 consecutive days were also studied. The chicken were slaughtered in different time and tissue samples were taken from liver, kidney, lungs, spleen, intestine, breast and thigh muscles to determined the concentration of both drugs in serum and tissues. The effect of both drugs on serum liver enzymes and the concentration of uric acid in serum were also studied. The bacterial sensitivity, the minimum inhibitory concentration and minimum bactericidal concentration of both drugs to Escherchia.coli O78, proteus mirabilis and pseudomonas aeroginosa were also investigated. 1-Cephradine 1-Single intravenous injection: After intravenous injection of cephradine in a dose of 50 mg kg-1 b.wt., the drug was showed a high serum level (38.67 ug ml-1) 5 min. post injection, then the concentration decreased gradually till reached its minimum level (0.474 ug ml-1) at 8 h post –injection. The present data revealed that the serum concentration time curves of cephradine were best described by a two compartment –open model. The drug was rapidly distributed with a distribution half-life (t 0.5 a) of 0.120 h and apparent volume of distribution calculated by extrapolation Vd(B), area Vd(area), pseudoequilibrium Vd (ß) and steady state Vd(ss) methods were 6.018, 3.102, 3.336 and 2.187 L/kg respectively. The drug was distributed in the central compartment with a volume of distribution (Vc) 0.836 L/kg., indicating that the drug is highly distributed in extra vascular tissues. The drug was rapidly eliminated with a half-life of elimination (t 0.5 ß) of 1.047 and the body clearance of cephradine (ClB)was 2.35 L/kg/h.The mean residence time(MRT) was 0.991h. 2-Single intramuscular injection: Following intramuscular injection of cephradine in a single dose of 50 mg kg-1 b.wt., the drug was detected in serum 0.083 h post injection (6.048ug ml-1) and it continued to increase gradually thereafter and was reached its maximum level 9.104 at 0.25 h post injection, then started to decrease gradually till reached its lowest concentration 0.34 ug ml-1 at 8 hours. It was rapidly absorbed with a half-life of absorption (t 0.5 ab )of 0.154 h., while its elimination half-life (t 0.5 el) was 0.859 h. .The systemic bioavailability of cephradine following intramuscular injection was 59.386 %. This indicated moderate absorption of the drug from this site of injection. While its in-vitro protein binding percent to serum was 10.03%. These values indicated that the drug is slightly bound to serum proteins. 3-Single subcutaneous injection: Following subcutaneous injection of cephradine in a single dose of 50 mg kg-1b.wt., the drug was detected in serum 0.083 h post injection (6.091ug ml-1) and it continued to increase gradually thereafter and was reached its maximum level 8.018 at 0.25 h post injection, then started to decrease gradually till reached its lowest concentration 0.281 ug ml-1 at 12 hours. It was rapidly absorbed with a half-life of absorption (t 0.5 ab) of 0.364 h., while its elimination half-life (t 0.5 el) was 2.652 h .The systemic bioavailability of cephradine following subcutaneous injection was 84.50 %. This indicated good absorption of the drug from this site of injection. 4-Single oral administration: Following oral administration of cephradine in a single dose of 50 mg kg-1 b.wt., the drug was detected in serum 0.167 h post injection (2.23 ug ml-1) and it continued to increase gradually thereafter and was reached its maximum level 5.33 ug ml-1 at 2 h post injection, then started to decrease gradually till reached its lowest concentration 0.557 ug ml-1 at 8 hours. It was rapidly absorbed with a half-life of absorption (t 0.5 ab) of 0.65 h., while its elimination half-life (t 0.5 el) was 1.74 h .The systemic bioavailability of cephradine following oral administration was 97.97%. This indicated good absorption of the drug from this site of injection. 5-Repeated oral administration : Multiple–dose studies have demonstrated no accumulation of cephradine serum level over 5 days with a 12 hours dosing regimen, where the concentrations of the drug in serum just before and one hour after each dose have been ranged from (0.912 to 1.72.ug ml-1) and ( 7.092 to 10.33ug ml-1)respectively . Cephradine was detected in examined tissues up to 12 h after last dose. Repeated oral administration of cephradine in a dose of 50 mg kg-1 b.wt. twice daily for 5 consecutive days caused no change in serum enzyme activity of SAST and SALT and significant increase in concentration of uric concentration at 72 to 120 hours of administration. 6- In –vitro activity of cephradine against certain pathogen: Escherchia.coli O78 was sensitive to cephradine while proteus mirabilis and pseudomonas aeroginosa were resistant. The minimum inhibitory concentrations of cephradine that prevent the growth of Escherchia.coli O78 , proteus mirabilis and pseudomonas aeroginosa were 32, 128 and more than128 while the minimum bactericidal concentration of the drug that kill them were 64, 128 and more than 128 ug ml-1, respectively. 2-Cefquinome 1-Single intramuscular injection: Following intramuscular injection of cefquinome in a single dose of 2 mg kg-1 b.wt., the drug was detected in serum 0.083 h post injection (6.786 ug ml-1) and it continued to increase gradually thereafter and was reached its maximum level 13.334 at 0.25 h post injection, then started to decrease gradually till reached its lowest concentration 0.525 ug ml-1 at 8 hours. It was rapidly absorbed with a half-life of absorption (t 0.5 ab) of 0.170 h., while its elimination half-life (t 0.5 el) was 3.428 h .While its in-vitro protein binding percent to serum was 6.665%. These values indicated that the drug is slightly bound to serum proteins. 2-Single subcutaneous injection: Following subcutaneous injection of cefquinome in a single dose of 2 mg kg-1 b.wt, the drug was detected in serum 0.083 h post injection (1.545 ug ml-1) and it continued to increase gradually thereafter and was reached its maximum level 5.708 at 15 minutes post injection, then started to decrease gradually till reached its lowest concentration 0.011 ug ml-1 at 24 hours. It was rapidly absorbed with a half-life of absorption (t 0.5 ab) of 0.262 h., while its elimination half-life (t 0.5 el) was 25.023 h. 3-Repeated intramuscular injection: Multiple –dose studies have demonstrated significant accumulation of cefquinome serum level over 5 days with a 24 hours dosing regimen, where the concentrations of the drug in serum just before and on hour after each dose have been ranged from (0.841 to 1.664.ug ml-1) and ( 6.96 to 17.31 ug ml-1)respectively . Cefquinome was detected in examined tissues up to 120 h after last dose . Repeated intramuscular injection of cefquinome in a dose of 2 mg kg-1 b.wt. once daily for 5 consecutive days have caused no change in serum enzyme activity of SAST and SALT and significant increase in concentration of uric concentration at 72 to 120 hours of administration. 4- In –vitro activity of cefquinome against certain pathogen: Escherchia.coli O78, proteus mirabilis and pseudomonas aeroginosa aeroginosa were sensitive to cefquinome. The minimum inhibitory concentrations of cephradine that prevent the growth of Escherchia.coli O78 , proteus mirabilis and pseudomonas aeroginosa were 0.5, 1 and 16 while the minimum bactericidal concentration of the drug that kill them were 1,16 and more than128ug ml-1, respectively. 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